ARG52438

anti-Synaptotagmin phospho (Ser309) antibody

anti-Synaptotagmin phospho (Ser309) antibody for Immunohistochemistry,Western blot and Rat

Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Synaptotagmin phospho (Ser309)
Tested Reactivity Rat
Predict Reactivity Hu, Ms, Bov, Chk, Dog, NHuPrm, Zfsh
Tested Application IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Synaptotagmin
Antigen Species Rat
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser309 conjugated to KLH
Conjugation Un-conjugated
Alternate Names P65; Synaptotagmin I; SytI; SVP65; SYT; p65; Synaptotagmin-1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC1:400
WB1:1000
Application Note Specific for the ~60k-62k synaptotagmin protein phosphorylated at Ser309
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25716 Rat SYT1

Swiss-port # P21707 Rat Synaptotagmin-1

Gene Symbol SYT1
Gene Full Name synaptotagmin I
Background Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin’s ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).
Research Area Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 48 kDa

Images (2) Click the Picture to Zoom In

  • ARG52438 anti-Synaptotagmin phospho (Ser309) antibody WB image

    Western blot: Rat cortex lysate showing specific immunolabeling of the ~62k synaptotagmin stained with ARG52438 anti-Synaptotagmin phospho (Ser309) antibody.

  • ARG52438 anti-Synaptotagmin phospho (Ser309) antibody IHC image

    Immunohistochemistry: 14 DIV Rat cortical neurons showing synaptotagmin when phosphorylated at Ser309 stained with ARG52438 anti-Synaptotagmin phospho (Ser309) antibody in red and PSD95 in green.