ARG20194

anti-Syndecan 4 antibody

anti-Syndecan 4 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Syndecan 4
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-Fr, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Syndecan 4
Antigen Species Human
Immunogen Synthetic peptide surrounding amino acid 184 of human Syndecan-4
Conjugation Un-conjugated
Alternate Names Amphiglycan; Ryudocan core protein; SYND4; Syndecan-4

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF5 - 10 µg/ml
IHC-Fr5 -25 µg/ml
IHC-P5 - 10 µg/ml
IP5 - 10 µg/ml
WB0.5 - 4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 22 kDa

Properties

Form Liquid
Purification Affinity Purified Antibody
Buffer PBS (pH 7.2), 30% Glycerol, 0.5% BSA and 0.01% Thimerosal
Preservative 0.01% Thimerosal
Stabilizer 30% Glycerol, 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 20971 Mouse SDC4

GeneID: 24771 Rat SDC4

GeneID: 6385 Human SDC4

Background Syndecans are type I integral membrane proteoglycans that are involved in cell-extracellular matrix adhesion and growth factor binding. Syndecan-1 is an matrix receptor which binds to Collagens, Fibronectin and Thrombospondin. Syndecan-1 and Syndecan-3 interact with MK (midkine), a growth/differentiation factor involved in embryogenesis. Syndecan-2 is highly expressed at areas of high morphogenetic activity, such as epithelial-mesenchymal interfaces and the prechondrogenic and preosteogenic mesenchymal condensations. Syndecan-4 functions cooperatively with integrins in the processes of cell spreading and focal adhesion assembly.

Images (1) Click the Picture to Zoom In

  • ARG20194 anti-Syndecan 4 antibody WB image

    Western blot: Jurkat cell lysate stained with ARG20194 anti-Syndecan 4 antibody.