ARG45787

anti-Syntaxin 12 / Syntaxin-13 antibody

anti-Syntaxin 12 / Syntaxin-13 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Syntaxin 12 / Syntaxin-13
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Syntaxin 12 / Syntaxin-13
Antigen Species Human
Immunogen Recombinant protein containing to human Syntaxin 12 / Syntaxin-13.
Conjugation Un-conjugated
Alternate Names STX12; Syntaxin 12; STX13; STX14; Syntaxin-12

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 37 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 100226 Mouse STX12

GeneID: 23673 Human STX12

GeneID: 65033 Rat STX12

Gene Symbol STX12
Gene Full Name Syntaxin 12
Background Predicted to enable SNAP receptor activity and SNARE binding activity. Involved in autophagosome assembly; cholesterol efflux; and protein stabilization. Located in several cellular components, including BLOC-1 complex; phagocytic vesicle; and phagophore assembly site. Part of SNARE complex. [provided by Alliance of Genome Resources, Feb 2025]
Function SNARE promoting fusion of transport vesicles with target membranes. Together with SNARE STX6, promotes movement of vesicles from endosomes to the cell membrane, and may therefore function in the endocytic recycling pathway. Through complex formation with GRIP1, GRIA2 and NSG1 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting. [UniProt]
Cellular Localization Endosome; Golgi apparatus; Membrane. [UniProt]
Calculated MW 32 kDa
PTM Acetylation; Phosphoprotein. [UniProt]

Images (5) Click the Picture to Zoom In

  • ARG45787 anti-Syntaxin 12 / Syntaxin-13 antibody ICC/IF image

    Immunofluorescence: Hela stained with ARG45787 anti-Syntaxin 12 / Syntaxin-13 antibody at 5 μg/ml dilution.

  • ARG45787 anti-Syntaxin 12 / Syntaxin-13 antibody WB image

    Western blot: MDA-MB-453 and 293T stained with ARG45787 anti-Syntaxin 12 / Syntaxin-13 antibody at 0.5 μg/ml dilution.

  • ARG45787 anti-Syntaxin 12 / Syntaxin-13 antibody FACS image

    Flow Cytometry: 293T stained with ARG45787 anti-Syntaxin 12 / Syntaxin-13 antibody at 1 µg/10^6 cells dilution.

  • ARG45787 anti-Syntaxin 12 / Syntaxin-13 antibody WB image

    Western blot: Rat brain stained with ARG45787 anti-Syntaxin 12 / Syntaxin-13 antibody at 0.5 μg/ml dilution.

  • ARG45787 anti-Syntaxin 12 / Syntaxin-13 antibody WB image

    Western blot: Mouse brain stained with ARG45787 anti-Syntaxin 12 / Syntaxin-13 antibody at 0.5 μg/ml dilution.