ARG52439

anti-Syntaxin antibody [SP8]

anti-Syntaxin antibody [SP8] for Immunohistochemistry,Western blot and Hamster,Human,Mouse,Pig,Rat

Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
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Overview

Product Description

Mouse Monoclonal antibody [SP8] recognizes Syntaxin

Tested Reactivity Hu, Ms, Rat, Hm, Pig
Tested Application IHC, WB
Host Mouse
Clonality Monoclonal
Clone SP8
Isotype IgG1
Target Name Syntaxin
Antigen Species Human
Immunogen Synaptic immunoprecipitate (crude) from human brain
Conjugation Un-conjugated
Alternate Names HPC-1; STX1; Syntaxin-1A; SYN1A; Neuron-specific antigen HPC-1; P35-1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC1:100
WB1:500 - 1:2000
Application Note The antibody is specific for the ~ 36 kDa syntaxin protein in Western blots of Rat brain lysate. The antibody has also been used for immunohistochemistry on formalin fixed, vibRatome sections. Does not work on paraffin sections.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 116470 Rat STX1A

GeneID: 20907 Mouse STX1A

GeneID: 6804 Human STX1A

Gene Symbol STX1A
Gene Full Name syntaxin 1A (brain)
Background Syntaxins are a family of proteins involved in the docking of synaptic vesicles with the plasma membrane. Syntaxin, along with synaptobrevin and SNAP-25, forms the SNARE complex which mediates synaptic vesicle fusion and exocytosis. Binding of Munc18-1 to the N-terminus of syntaxin 1 has been demonstrated to be essential for exocytic membrane fusion (Khvotchev et al., 2007). Decreased levels of phosphorylated syntaxin 1 in the prefrontal cortex have recently been correlated with schizophrenia (Castillo et al., 2010).
Research Area Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 33 kDa
PTM Phosphorylated by CK2 (By similarity). Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1.

Images (1) Click the Picture to Zoom In

  • ARG52439 anti-Syntaxin antibody [SP8] WB image

    Western Blot: rat cortex lysate showing specific immunolabeling of the ~ 36k syntaxin 1A protein stained with Syntaxin antibody [SP8] (ARG52439).

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Review for anti-Syntaxin antibody [SP8]

Application:WB

Sample:Mouse brain and Rat brain

Sample Loading Amount:20 µl

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Clone References

Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex.

Honer WG et al.
Brain Res.,  (1993)

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