ARG45596

anti-T Plastin / PLS3 antibody

anti-T Plastin / PLS3 antibody for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes T Plastin / PLS3
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name T Plastin / PLS3
Antigen Species Human
Immunogen Recombinant protein containing to human T Plastin / PLS3.
Conjugation Un-conjugated
Alternate Names PLS3; Plastin 3; T-Plastin; Plastin-3; Plastin 3 (T Isoform); T Fimbrin; T Plastin; T-Fimbrin; BMND18; DIH5

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
IP2-4 μg/ml
WB0.1-0.25 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 71 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 102866 Mouse PLS3

GeneID: 5358 Human PLS3

Swiss-port # P13797 Human Plastin-3

Swiss-port # Q99K51 Mouse Plastin-3

Gene Symbol PLS3
Gene Full Name Plastin 3
Background Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]
Function Actin-bundling protein. [UniProt]
Cellular Localization Cytoplasm; Nucleus. [UniProt]
Calculated MW 71 kDa
PTM Phosphoprotein. [UniProt]

Images (6) Click the Picture to Zoom In

  • ARG45596 anti-T Plastin / PLS3 antibody ICC/IF image

    Immunofluorescence: A549 stained with ARG45596 anti-T Plastin / PLS3 antibody at 5 μg/ml dilution.

  • ARG45596 anti-T Plastin / PLS3 antibody WB image

    Western blot: Hela and A549 stained with ARG45596 anti-T Plastin / PLS3 antibody at 0.5 μg/ml dilution.

  • ARG45596 anti-T Plastin / PLS3 antibody IP image

    Immunoprecipitation: Hela lysate immunoprecipitated with 2 μg ARG45596 anti-T Plastin / PLS3 antibody.

  • ARG45596 anti-T Plastin / PLS3 antibody FACS image

    Flow Cytometry: PC-3 stained with ARG45596 anti-T Plastin / PLS3 antibody at 1 µg/10^6 cells dilution.

  • ARG45596 anti-T Plastin / PLS3 antibody WB image

    Western blot: Rat stomach stained with ARG45596 anti-T Plastin / PLS3 antibody at 0.5 μg/ml dilution.

  • ARG45596 anti-T Plastin / PLS3 antibody WB image

    Western blot: Mouse stomach stained with ARG45596 anti-T Plastin / PLS3 antibody at 0.5 μg/ml dilution.