ARG54684

anti-TAB1 antibody

anti-TAB1 antibody for ELISA,Western blot,ICC/IF and Human,Mouse

Cell Biology and Cellular Response antibody; Immune System antibody; Signaling Transduction antibody
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Overview

Product Description Rabbit Polyclonal antibody recognizes TAB1
Tested Reactivity Hu, Ms
Tested Application ELISA, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TAB1
Immunogen Synthetic peptide (13 aa) within aa. 220-270 of Human TAB1.
Conjugation Un-conjugated
Alternate Names MAP3K7IP1; Mitogen-activated protein kinase kinase kinase 7-interacting protein 1; TGF-beta-activated kinase 1 and MAP3K7-binding protein 1; 3'-Tab1; TGF-beta-activated kinase 1-binding protein 1; TAK1-binding protein 1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IF1 - 2 μg/ml
WB0.5 - 2 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control 3T3 Cell Lysate

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10454 Human TAB1

GeneID: 66513 Mouse TAB1

Swiss-port # Q15750 Human TGF-beta-activated kinase 1 and MAP3K7-binding protein 1

Swiss-port # Q8CF89 Mouse TGF-beta-activated kinase 1 and MAP3K7-binding protein 1

Gene Symbol TAB1
Gene Full Name TGF-beta activated kinase 1/MAP3K7 binding protein 1
Background TAB1 was identified as a regulator of the MAP kinase kinase kinase TAK1/MAP3K7, which is known to mediate various intracellular signaling pathways, such as those induced by TGF-beta and members of the Toll-IL-1R (TIR) superfamily, thus acting as an intermediate in both proliferative and innate and adaptive immune responses. This protein, together with either TAB2 or TAB3, activates TAK1 kinase in response to upstream signals. It has been shown that the C-terminal portion of TAB1 is sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF-beta, demonstrating how this protein can function as a mediator between TGF-beta receptors and TAK1.
Function May be an important signaling intermediate between TGFB receptors and MAP3K7/TAK1. May play an important role in mammalian embryogenesis. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 55 kDa
PTM Monoubiquitinated. Deubiquitinated by Y.enterocolitica YopP.

Images (3) Click the Picture to Zoom In

  • ARG54684 anti-TAB1 antibody ICC/IF image

    Immunocytochemistry: K562 cells stained with ARG54684 anti-TAB1 antibody at 1 μg/ml.

  • ARG54684 anti-TAB1 antibody ICC/IF image

    Immunofluorescence: 3T3 cells stained with ARG54684 anti-TAB1 antibody at 2 μg/ml.

  • ARG54684 anti-TAB1 antibody WB image

    Western blot: 3T3 cell lysate stained with ARG54684 anti-TAB1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/ml.

Customer's Feedback

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Review for anti-TAB1 antibody

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC