ARG45583

anti-TAF8 / TBN antibody

anti-TAF8 / TBN antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes TAF8 / TBN
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TAF8 / TBN
Antigen Species Human
Immunogen Recombinant protein containing to human TAF8 / TBN.
Conjugation Un-conjugated
Alternate Names TAF8; TATA-Box Binding Protein Associated Factor 8; TAF(II)43; TBN; TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase II, A, 45/50kDa; TAF8 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 43kDa; Transcription Initiation Factor TFIID 43 KDa Subunit; Transcription Initiation Factor TFIID Subunit 8; TBP-Associated Factor 43 KDa; TBP-Associated Factor 8; Protein Taube Nuss; FLJ32821; TAFII-43; HTAFII43; TAFII43; TBP-Associated Factor, RNA Polymerase II, 43 KD; TBP-Associated Factor TAFII43; Taube Nuss Homolog (Mouse); Taube Nuss Homolog; NEDMLHB; TAF

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 34 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 129685 Human TAF8

GeneID: 63856 Mouse TAF8

Swiss-port # Q7Z7C8 Human Transcription initiation factor TFIID subunit 8

Swiss-port # Q9EQH4 Mouse Transcription initiation factor TFIID subunit 8

Gene Symbol TAF8
Gene Full Name TATA-Box Binding Protein Associated Factor 8
Background This gene encodes one of several TATA-binding protein (TBP)-associated factors (TAFs), which are integral subunits of the general transcription factor complex TFIID. TFIID recognizes the core promoter of many genes and nucleates the assembly of a transcription preinitiation complex containing RNA polymerase II and other initiation factors. The protein encoded by this gene contains an H4-like histone fold domain, and interacts with several subunits of TFIID including TBP and the histone-fold protein TAF10. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
Function The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. [UniProt]
Cellular Localization Cytoplasm; Nucleus. [UniProt]
Calculated MW 34 kDa
PTM Acetylation; Phosphoprotein. [UniProt]

Images (5) Click the Picture to Zoom In

  • ARG45583 anti-TAF8 / TBN antibody ICC/IF image

    Immunofluorescence: CACO-2 stained with ARG45583 anti-TAF8 / TBN antibody at 5 μg/ml dilution.

  • ARG45583 anti-TAF8 / TBN antibody WB image

    Western blot: Hela stained with ARG45583 anti-TAF8 / TBN antibody at 0.5 μg/ml dilution.

  • ARG45583 anti-TAF8 / TBN antibody WB image

    Western blot: Rat heart stained with ARG45583 anti-TAF8 / TBN antibody at 0.5 μg/ml dilution.

  • ARG45583 anti-TAF8 / TBN antibody FACS image

    Flow Cytometry: A431 stained with ARG45583 anti-TAF8 / TBN antibody at 1 µg/10^6 cells dilution.

  • ARG45583 anti-TAF8 / TBN antibody WB image

    Western blot: Mouse heart stained with ARG45583 anti-TAF8 / TBN antibody at 0.5 μg/ml dilution.