anti-TCF3 / ITF1 antibody
anti-TCF3 / ITF1 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human
Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
|Product Description||Goat Polyclonal antibody recognizes TCF3 / ITF1|
|Tested Application||IHC-P, WB|
|Target Name||TCF3 / ITF1|
|Alternate Names||TCF-3; E47; VDIR; Class B basic helix-loop-helix protein 21; Immunoglobulin transcription factor 1; Transcription factor 3; Kappa-E2-binding factor; bHLHb21; E2A; Transcription factor ITF-1; Transcription factor E2-alpha; ITF1; Immunoglobulin enhancer-binding factor E12/E47|
|Application Note||WB: Recommend incubate at RT for 1h. Approx 75 kDa band observed in lysates of cell line Daudi (calculated MW of 67.6 kDa according to NP_003191.1). Recommended concentration: 0.01-0.03 µg/ml.
IHC-P: In paraffin embedded Human Kidney shows strong nuclear staining in DCT cells. Recommended concentration, 3-5 µg/ml.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Buffer||Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA|
|Preservative||0.02% Sodium azide|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Background||This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, Sep 2011]|
|Research Area||Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody|
|PTM||Phosphorylated following NGF stimulation.|
Images (2) Click the Picture to Zoom In
ARG64126 anti-TCF3 / ITF1 antibody WB image
Western Blot: Daudi cell lysate (35 µg protein in RIPA buffer) stained with ARG64126 anti-TCF3 antibody at 0.01 µg/ml dilution.
ARG64126 anti-TCF3 / ITF1 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Kidney. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64126 anti-TCF3 antibody at 3.8 µg/ml dilution followed by AP-staining.