ARG64126

anti-TCF3 / ITF1 antibody

anti-TCF3 / ITF1 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human

Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes TCF3 / ITF1
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name TCF3 / ITF1
Antigen Species Human
Immunogen C-KAPRARTSPDEDED
Conjugation Un-conjugated
Alternate Names TCF-3; E47; VDIR; Class B basic helix-loop-helix protein 21; Immunoglobulin transcription factor 1; Transcription factor 3; Kappa-E2-binding factor; bHLHb21; E2A; Transcription factor ITF-1; Transcription factor E2-alpha; ITF1; Immunoglobulin enhancer-binding factor E12/E47

Application Instructions

Application Note WB: Recommend incubate at RT for 1h. Approx 75 kDa band observed in lysates of cell line Daudi (calculated MW of 67.6 kDa according to NP_003191.1). Recommended concentration: 0.01-0.03 µg/ml.
IHC-P: In paraffin embedded Human Kidney shows strong nuclear staining in DCT cells. Recommended concentration, 3-5 µg/ml.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 6929 Human TCF3

Swiss-port # P15923 Human Transcription factor E2-alpha

Background This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, Sep 2011]
Research Area Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
PTM Phosphorylated following NGF stimulation.

Images (2) Click the Picture to Zoom In

  • ARG64126 anti-TCF3 / ITF1 antibody WB image

    Western Blot: Daudi cell lysate (35 µg protein in RIPA buffer) stained with ARG64126 anti-TCF3 antibody at 0.01 µg/ml dilution.

  • ARG64126 anti-TCF3 / ITF1 antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Kidney. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64126 anti-TCF3 antibody at 3.8 µg/ml dilution followed by AP-staining.