ARG21174

anti-TCR alpha + TCR beta antibody [TCR-3] (FITC)

anti-TCR alpha + TCR beta antibody [TCR-3] (FITC) for Flow cytometry,IHC-Frozen sections,Depletion and Chicken

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [TCR-3] recognizes TCR alpha + TCR beta
Tested Reactivity Chk
Tested Application Depletion, FACS, IHC-Fr
Specificity The clone TCR-3 precipitates a CD3-associated heterodimer of 88 kDa (two bands of 48 kDa and 40 kDa upon reduction) on chicken peripheral blood T cells. Deglycosylation of the heterodimer yields two polypeptides of 34 kDa and 31 kDa.
Host Mouse
Clonality Monoclonal
Clone TCR-3
Isotype IgG1, kappa
Target Name TCR alpha + TCR beta
Antigen Species Chicken
Immunogen CD3+ TCR1- TCR2- Ia- chicken blood mononuclear cells
Conjugation FITC
Full Name T cell receptor alpha locus; T cell receptor beta locus
Alternate Names TCR alpha: IMD7; TCRA
TCR beta: TCRB

Application Instructions

Application Suggestion
Tested Application Dilution
DepletionAssay-dependent
FACS< 1 µg/10^6 cells
IHC-FrAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS and Sodium azide
Preservative Sodium azide
Concentration 0.5 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol TRA; TRB
Gene Full Name T cell receptor alpha locus; T cell receptor beta locus
Background TCR beta: T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Several V segments and one J segment of the beta locus are known to be incapable of encoding a protein and are considered pseudogenes. The beta locus also includes eight trypsinogen genes, three of which encode functional proteins and five of which are pseudogenes. Chromosomal abnormalities involving the T-cell receptor beta locus have been associated with T-cell lymphomas. [provided by RefSeq, Jul 2008]