ARG52443

anti-TDP43 antibody

anti-TDP43 antibody for Western blot,Immunohistochemistry and Human,Rat

Gene Regulation antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes TDP43
Tested Reactivity Hu, Rat
Tested Application IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TDP43
Antigen Species Human
Immunogen Synthetic peptide C-terminal to the caspase-cleavage site (between D219 and V220) of human TDP-43.
Conjugation Un-conjugated
Alternate Names TAR DNA-binding protein 43; TDP-43; ALS10

Application Instructions

Application Suggestion
Tested Application Dilution
IHC1:250
WB1:1000
Application Note Specific for the ~ 43kDa TDP-43 protein in Western blots of Rat brain lysate. Expected molecular weight is dependent upon the TDP-43 species present in sample (full-length vs. truncated TDP-43). Under non-denaturing conditions (for example, by IHC), this antibody detects TDP-43 inclusions in human brain tissue with TDP-43 proteinopathy, but does not detect full-length nuclear TDP-43. Under denaturing conditions (for example, by Western Blot analysis) this antibody detects the C-terminus of full-length and truncated human TDP-43.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer PBS and 30% Glycerol
Stabilizer 30% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23435 Human TARDBP

Swiss-port # Q13148 Human TAR DNA-binding protein 43

Gene Symbol TARDBP
Gene Full Name TAR DNA binding protein
Background TDP43 (Tar DNA Binding 43, TARDBP) was originally identified as a protein which binds to the "transactivation response" (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome (Ou et al.,1995). UV cross-linking of HeLa cell extract revealed a 43kDa protein which was cloned and sequenced and shown to contain two copies of the ~90 amino acid RRM domain. RRM is an acronym for RNA Recognition Motif, and this domain is found in many proteins which bind single stranded RNA and some which bind single stranded DNA. Northern blots showed that the protein is ubiquitous in tissue expression. Much interest has been focused on TDP43 recently due to its association with the inclusions seen in frontotemporal lobar degeneration and Amyotrophic Lateral Sclerosis (Neumann et al., 2006). The protein is present in these inclusions in partially degraded, hyperphosphorylated and ubiquitinated forms.
Research Area Gene Regulation antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody
Calculated MW 45 kDa
PTM Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.

Images (1) Click the Picture to Zoom In

  • ARG52443 anti-TDP43 antibody WB image

    Western blot: Rat hippocampal lysate showing specific immunolabeling of the ~58k TR-α2 protein stained with ARG52443 anti-TDP43 antibody.