ARG52444

anti-TFAM antibody

anti-TFAM antibody for Western blot and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes TFAM
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TFAM
Antigen Species Mouse
Immunogen Native recombinant mouse TFAM protein with c-terminal 6-his tag
Conjugation Un-conjugated
Alternate Names TCF6; MTTFA; mtTFA; TCF-6; Transcription factor A, mitochondrial; MtTF1; MTTF1; Mitochondrial transcription factor 1; Transcription factor 6-like 2; TCF6L1; Transcription factor 6; TCF6L3; TCF6L2

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:2000
Application Note Specific for the ~24 kDa TFAM protein.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Neat Serum
Buffer Neat serum
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21780 Mouse TFAM

GeneID: 7019 Human TFAM

GeneID: 83474 Rat TFAM

Gene Symbol TFAM
Gene Full Name transcription factor A, mitochondrial
Background Mitochondrial Transcription Factor A (TFAM) is a key activator of mitochondrial (mt) DNA transcription as well as a participant in mitochondrial genome replication. mtDNA is highly susceptible to oxidative stress leading to mitochondrial dysfunction. Overexpression of TFAM has been implicated in the amelioration of age dependent impairment of brain functions through the prevention of oxidative stress and mitochondrial dysfunction in microglia (Hayashi et al., 2008). More recently, TFAM overexpression has been shown to potentially reduce oxidative stress in motor neurons and delay onset of amyotrophic lateral sclerosis (ALS) in ALS model mice (Morimoto et al., 2012).
Research Area Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 29 kDa
PTM Phosphorylation by PKA within the HMG box 1 impairs DNA binding and promotes degradation by the AAA+ Lon protease.

Images (1) Click the Picture to Zoom In

  • ARG52444 anti-TFAM antibody WB image

    Western blot: Rat kidney lysate showing specific immunolabeling of the ~24 kDa TFAM protein stained with ARG52444 anti-TFAM antibody.