ARG54637

anti-TFIIB antibody [IIB8]

anti-TFIIB antibody [IIB8] for ELISA,Western blot,Electrophoretic mobility shift assay and Human,Mouse

Gene Regulation antibody

Overview

Product Description Mouse Monoclonal antibody [IIB8] recognizes TFIIB
Tested Reactivity Hu, Ms
Tested Application ELISA, EMSA, WB
Specificity This antibody recognizes an epitopewithin amino acids 52-105 of humanand mouse TFIIB.
Host Mouse
Clonality Monoclonal
Clone IIB8
Isotype IgG2a
Target Name TFIIB
Antigen Species Human
Immunogen Partially purified recombinanthuman transcription factor IIB (
Conjugation Un-conjugated
Alternate Names Transcription initiation factor IIB; General transcription factor TFIIB; TF2B; TFIIB; S300-II

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
EMSAAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity chromatography
Buffer PBS (pH 7.4)
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 229906 Mouse GTF2B

GeneID: 2959 Human GTF2B

Swiss-port # P62915 Mouse Transcription initiation factor IIB

Swiss-port # Q00403 Human Transcription initiation factor IIB

Gene Symbol GTF2B
Gene Full Name general transcription factor IIB
Background This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008]
Function General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II. [UniProt]
Research Area Gene Regulation antibody
Calculated MW 35 kDa

Clone References

Protection against oxidative stress through SUA7/TFIIB regulation in Saccharomyces cerevisiae.

WB / Saccharomyces cerevisiae

Paes de Faria J et al.
Free Radic Biol Med.,  (2006)

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Purification of recombinant human transcription factor IIB by immunoaffinity chromatography.

Thompson NE et al.
Protein Expr Purif.,  (1994)

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Accessibility of epitopes on human transcription factor IIB in the native protein and in a complex with DNA.

Thompson NE et al.
J Biol Chem.,  (1995)

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