ARG58890
anti-TFIIE antibody
anti-TFIIE antibody for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TFIIE |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TFIIE |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 230-439 of Human TFIIE (NP_005504.2). |
| Conjugation | Un-conjugated |
| Alternate Names | General transcription factor IIE subunit 1; General transcription factor IIE 56 kDa subunit; TF2E1; TFIIE-alpha; FE; TFIIE-A; Transcription initiation factor IIE subunit alpha |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HeLa | ||||||
| Observed Size | 60 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P29083 Human General transcription factor IIE subunit 1 Swiss-port # Q9D0D5 Mouse General transcription factor IIE subunit 1 |
|---|---|
| Gene Symbol | GTF2E1 |
| Gene Full Name | general transcription factor IIE, polypeptide 1, alpha 56kDa |
| Function | Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 49 kDa |
Images (1) Click the Picture to Zoom In
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ARG58890 anti-TFIIE antibody WB image
Western blot: 25 µg of HeLa cell lysate stained with ARG58890 anti-TFIIE antibody at 1:1000 dilution.
