anti-TGF beta antibody [1D11.16.8]

anti-TGF beta antibody [1D11.16.8] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Cow,Dog,Hamster,Human,Monkey,Mouse,Rat

publication_link Publication1


Product Description Mouse Monoclonal antibody [1D11.16.8] recognizes TGF beta
Tested Reactivity Hu, Ms, Rat, Cow, Dog, Hm, Mk
Tested Application ELISA, ICC/IF, IHC-P, Neut, WB
Specificity This mAb recognizes TGF beta 1, 2 and 3.
Host Mouse
Clonality Monoclonal
Clone 1D11.16.8
Isotype IgG1, kappa
Target Name TGF beta
Antigen Species Bovine
Immunogen Bovine TGF-beta2 protein.
Conjugation Un-conjugated
Alternate Names TGF-beta2; Cetermin; LDS4; Polyergin; Glioblastoma-derived T-cell suppressor factor; G-TSF; Transforming growth factor beta-2; LAP; TGF-beta-2; BSC-1 cell growth inhibitor

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA0.1-0.4 μg/ml (detect); 2-8 μg/ml (capture)
ICC/IF8-25 μg/ml
IHC-P8-25 μg/ml
WB1-2 μg/ml (under non-reducing conditions only)
Application Note WB: It is recommended detct TGF beta under non-reducing conditions.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 21808 Mouse TGFB2

GeneID: 7042 Human TGFB2

GeneID: 81809 Rat TGFB2

Gene Symbol TGFB2
Gene Full Name transforming growth factor, beta 2
Background This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types by transducing their signal through combinations of transmembrane type I and type II receptors (TGFBR1 and TGFBR2) and their downstream effectors, the SMAD proteins. Disruption of the TGFB/SMAD pathway has been implicated in a variety of human cancers. The encoded protein is secreted and has suppressive effects of interleukin-2 dependent T-cell growth. Translocation t(1;7)(q41;p21) between this gene and HDAC9 is associated with Peters' anomaly, a congenital defect of the anterior chamber of the eye. The knockout mice lacking this gene show perinatal mortality and a wide range of developmental, including cardiac, defects. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010]
Function TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. [UniProt]
Cellular Localization Cytoplasm and extracellular (secreted)
Calculated MW 48 kDa
PTM The precursor is cleaved into mature TGF-beta-2 and LAP, which remains non-covalently linked to mature TGF-beta-2 rendering it inactive.

Specific References

Mouse Astrocytes Promote Microglial Ramification by Releasing TGF-β and Forming Glial Fibers.

Neutralizing / Mouse

Jinqiang Zhang et al.
Front Cell Neurosci.,  (2020)