anti-TGFBI antibody

anti-TGFBI antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

publication_link Publication4


Product Description Rabbit Polyclonal antibody recognizes TGFBI
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TGFBI
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 481-683 of Human TGFBI (NP_000349.1).
Conjugation Un-conjugated
Alternate Names CDGG1; LCD1; RGD-CAP; CSD2; CSD; Beta ig-h3; CSD1; Transforming growth factor-beta-induced protein ig-h3; RGD-containing collagen-associated protein; BIGH3; CDG2; CSD3; Kerato-epithelin; CDB1; EBMD

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Rat kidney, Mouse heart and BxPC3
Observed Size ~ 74 kDa


Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 21810 Mouse TGFBI

GeneID: 7045 Human TGFBI

Swiss-port # P82198 Mouse Transforming growth factor-beta-induced protein ig-h3

Swiss-port # Q15582 Human Transforming growth factor-beta-induced protein ig-h3

Gene Symbol TGFBI
Gene Full Name transforming growth factor, beta-induced, 68kDa
Background This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008]
Function Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation. [UniProt]
Cellular Localization Secreted. Secreted, extracellular space, extracellular matrix. Note=May be associated both with microfibrils and with the cell surface (PubMed:8077289). [UniProt]
Calculated MW 75 kDa
PTM Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; these residues may be required for binding to calcium (PubMed:18450759). According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues (PubMed:26273833).

The EMI domain contains 2 expected intradomain disulfide bridges (Cys-49-Cys85 and Cys-84-Cys-97) and one unusual interdomain disulfide bridge to the second FAS1 domain (Cys-74-Cys-339). This arrangement violates the predicted disulfide bridge pattern of an EMI domain. [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG56894 anti-TGF beta 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung cancer stained with ARG56894 anti-TGF beta 1 antibody at 1:100 dilution.

  • ARG56894 anti-TGF beta 1 antibody WB image

    Western blot: 25 µg of Rat kidney, Mouse heart and BxPC-3 cell lysates stained with ARG56894 anti-TGF beta 1 antibody at 1:1000 dilution.

  • ARG56894 anti-TGF beta 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colon carcinoma stained with ARG56894 anti-TGF beta 1 antibody at 1:100 dilution.

Specific References

Transcriptomic analysis identifies upregulation of secreted phosphoprotein 1 in silicotic rats

WB / Rat

Wenchen Cai et al.
Exp Ther Med.,  (2021)




MicroRNA-411-3p inhibits bleomycin-induced skin fibrosis by regulating transforming growth factor-β/Smad ubiquitin regulatory factor-2 signalling

WB / Mouse

Ziyan Zhang et al.
J Cell Mol Med.,  (2021)




MiR-411-3p alleviates Silica-induced pulmonary fibrosis by regulating Smurf2/TGF-β signaling.

WB, IHC-P / Mouse

Gao Xuemin et al.
Exp Cell Res.,  (2020)




Inhibition of miR-155-5p Exerts Anti-Fibrotic Effects in Silicotic Rats by Regulating Meprin α

WB / Rat

Chen Ying-Ying et al.
Preprint.,  (2019)