ARG10593

anti-TGM2 / Tissue transglutaminase antibody

anti-TGM2 / Tissue transglutaminase antibody for IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human,Mouse,Rat

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anti-TGM2 / Tissue transglutaminase antibody

Rabbit Polyclonal antibody recognizes TGM2 / Tissue transglutaminase

ARG10593 Arigo biolaboratories
3.0 out of 5 based on 1
anti-TGM2 / Tissue transglutaminase antibody is rated 3.0 out of 5 by 1.

anti-TGM2 / Tissue transglutaminase antibody

Overview

Product Description

Rabbit Polyclonal antibody recognizes TGM2 / Tissue transglutaminase

Tested Reactivity Hu, Ms, Rat
Tested Application IHC-Fr, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TGM2 / Tissue transglutaminase
Antigen Species Human
Immunogen Synthetic peptide derived from Human TGM2 / Tissue transglutaminase protein: LERCDLELETNGRDHHTADLCREKLVVR
Conjugation Un-conjugated
Full Name transglutaminase 2
Alternate Names TG2; G-ALPHA-h; TGase H; Transglutaminase C; TGase C; HEL-S-45; Transglutaminase-2; Tissue transglutaminase; C; TGase-2; TGC; Protein-glutamine gamma-glutamyltransferase 2; TG; Transglutaminase H; GNAH; EC 2.3.2.13

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-Fr0.5 - 1 µg/ml
IHC-P0.5 - 1 µg/ml
WB0.5 - 1 µg/ml
Application Note Antigen retrieval for IHC-P: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 78 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 1X PBS, 0.025% Sodium azide and 2.5% BSA
Preservative 0.025% Sodium azide
Stabilizer 2.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 21817 Mouse TGM2

GeneID: 7052 Human TGM2

Swiss-port # P21980 Human Protein-glutamine gamma-glutamyltransferase 2

Swiss-port # P21981 Mouse Protein-glutamine gamma-glutamyltransferase 2

Gene Symbol TGM2
Gene Full Name transglutaminase 2
Background Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. [UniProt]

Images (8) Click the Picture to Zoom In

  • ARG10593 anti-TGM2 antibody IHC-P image

    Immunohistochemistry: Human intestine cancer stained with ARG10593 anti-TGM2 antibody.

  • ARG10593 anti-TGM2 antibody WB image

    Western blot: 1) Rat liver, 2) Rat ovary, and 3) HeLa cell lysate stained with ARG10593 anti-TGM2 antibody.

  • ARG10593 anti-TGM2 antibody IHC-P image

    Immunohistochemistry: Human breast cancer stained with ARG10593 anti-TGM2 antibody.

  • ARG10593 anti-TGM2 antibody IHC-Fr image

    Immunohistochemistry: Human placental tissue stained with ARG10593 anti-TGM2 antibody.

  • ARG10593 anti-TGM2 antibody IHC-P image

    Immunohistochemistry: Mouse spleen tissue stained with ARG10593 anti-TGM2 antibody.

  • ARG10593 anti-TGM2 antibody IHC-Fr image

    Immunohistochemistry: Mouse spleen tissue stained with ARG10593 anti-TGM2 antibody.

  • ARG10593 anti-TGM2 antibody IHC-P image

    Immunohistochemistry: Rat spleen tissue stained with ARG10593 anti-TGM2 antibody.

  • ARG10593 anti-TGM2 antibody IHC-Fr image

    Immunohistochemistry: Rat spleen tissue stained with ARG10593 anti-TGM2 antibody.

Customer's Feedback

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Review for anti-TGM2 / Tissue transglutaminase antibody

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC