anti-TIM3 antibody [SQab22265]

anti-TIM3 antibody [SQab22265] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Immune System antibody


Product Description Recombinant Rabbit Monoclonal antibody [SQab22265] recognizes TIM3
Tested Reactivity Hu
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SQab22265
Isotype IgG
Target Name TIM3
Antigen Species Human
Immunogen A synthetic peptide corresponding to the sequence of Human TIM-3.
Conjugation Un-conjugated
Protein Full Name Hepatitis A virus cellular receptor 2
Alternate Names KIM-3; TIM3; TIMD-3; Tim-3; TIM-3; CD366; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD3; T-cell membrane protein 3; HAVcr-2; T-cell immunoglobulin mucin receptor 3; Hepatitis A virus cellular receptor 2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:1000 - 1:3000
IHC-P1:100 - 1:200
WB1:100 - 1:200 (5 x 10^5 cells)
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human tonsil


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05%BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 84868 Human HAVCR2

Swiss-port # Q8TDQ0 Human Hepatitis A virus cellular receptor 2

Gene Symbol HAVCR2
Gene Full Name hepatitis A virus cellular receptor 2
Background The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011]
Function Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9. [UniProt]
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Research Area Immune System antibody
Calculated MW 33 kDa
PTM O-glycosylated with core 1 or possibly core 8 glycans.
Phosphorylated on tyrosine residues; modestly increased after TCR/CD28 stimulation. Can be phosphorylated in the cytoplasmatic domain by FYN (By similarity). Phosphorylation at Tyr-265 is increased by stimulation with ligand LGALS9.

Images (3) Click the Picture to Zoom In

  • ARG66922 anti-TIM3 antibody [SQab22265] WB image (Customer's Feedback)

    Western blot: NK92-MI; TIM3 KD NK92-MI and Primary T cell stained with ARG66922 anti-TIM3 antibody [SQab22265] at 1:2000 dilution for 1 hour at room temperature.

  • ARG66922 anti-TIM3 antibody [SQab22265] FACS image (Customer's Feedback)

    Flow Cytometry: NK92-MI; TIM3 KD NK92-MI and Primary T cell stained with ARG66922 anti-TIM3 antibody [SQab22265] at 1:200 dilution for 50 min at 4ºC.

  • ARG66922 anti-TIM3 antibody [SQab22265] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human tonsil stained with ARG66922 anti-TIM3 antibody [SQab22265]. Antigen Retrieval: Heat tissue section in Tris-EDTA buffer (pH 9.0).

Customer's Feedback

nuts_pic      Excellent

Review for anti-TIM3 antibody [SQab22265]


Sample:NK92-MI; TIM3 KD NK92-MI; Primary T cell

Sample Loading Amount:35 μg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:1 h

Primary Antibody Incubation Temperature:Room Temperature ºC

nuts_pic      Excellent

Review for anti-TIM3 antibody [SQab22265]


Sample:Primary T cell; NK92-MI; TIM3 KD NK92-MI

Cell Number/Concentration:5x10^5 cells

Fixation Buffer:-

Fixation Time:-

Fixation Temperature:- ºC

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:50 min

Primary Antibody Incubation Temperature:4 ºC