ARG20584

anti-TIMP2 antibody [3A4]

anti-TIMP2 antibody [3A4] for Western blot,IHC-Formalin-fixed paraffin-embedded sections,IHC-Whole mount and Human,Zebrafish

Overview

Product Description Mouse Monoclonal antibody [3A4] recognizes TIMP2
Tested Reactivity Hu, Zfsh
Predict Reactivity Ms, Rat, Chk, Pig, Rb
Tested Application IHC-P, IHC-Wmt, WB
Host Mouse
Clonality Monoclonal
Clone 3A4
Isotype IgG2a
Target Name TIMP2
Species Human
Immunogen Synthetic peptide from Human TIMP-2 protein and Mouse myeloma Ag8563 cells.
Conjugation Un-conjugated
Full Name TIMP metallopeptidase inhibitor 2
Alternate Names TIMP-2; Tissue inhibitor of metalloproteinases 2; CSC-21K; DDC8; Metalloproteinase inhibitor 2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1-5 µg/ml
IHC-WmtAssay-dependent
WB1-2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control IHC: formalin-fixed, paraffin-embedded colonic adenocarcinoma.
Western blot: rhTIMP-2, 400 ng per lane.
Calculated MW 24 kDa

Properties

Form Liquid
Purification Purification with Protein A/G.
Buffer PBS and 0.08% Sodium azide
Preservative 0.08% Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 7077 Human TIMP2

Swiss-port # P16035 Human Metalloproteinase inhibitor 2

Gene Symbol TIMP2
Gene Full Name TIMP metallopeptidase inhibitor 2
Background This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008]
Function Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. [UniProt]
PTM The activity of TIMP2 is dependent on the presence of disulfide bonds.

Images (3) Click the Picture to Zoom In

  • ARG20584 anti-TIMP2 antibody [3A4] WB image

    Western blot: 400 ng of 1) TIMP-1, and 2) TIMP-2 recombinant protein stained with ARG20584 anti-TIMP2 antibody [3A4].

  • ARG20584 anti-TIMP2 antibody [3A4] IHC-P image

    Immunohistochemistry: wax-embedded Human colon stained with ARG20584 anti-TIMP2 antibody [3A4] at 1:32 dilution.

  • ARG20584 anti-TIMP2 antibody [3A4] IHC-Wmt image

    Immunohistochemistry: whole-mount Zebrafish embryo stained with ARG20584 anti-TIMP2 antibody [3A4].