anti-TLE1 antibody [SQab22275]

anti-TLE1 antibody [SQab22275] for IHC-Formalin-fixed paraffin-embedded sections and Human


Product Description Recombinant Rabbit Monoclonal antibody [SQab22275] recognizes TLE1
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SQab22275
Isotype IgG
Target Name TLE1
Antigen Species Human
Immunogen Full length protein corresponding to Human TLE1.
Conjugation Un-conjugated
Protein Full Name Transducin-like enhancer protein 1
Alternate Names ESG; ESG1; GRG1; TLE-1; Enhancer of split groucho-like protein 1; E(Sp1) homolog; Transducin-like enhancer protein 1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human synovial sarcoma


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05%BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 7088 Human TLE1

Swiss-port # Q04724 Human Transducin-like enhancer protein 1

Gene Symbol TLE1
Gene Full Name TLE family member 1, transcriptional corepressor
Background Enables identical protein binding activity and transcription corepressor activity. Involved in negative regulation of I-kappaB kinase/NF-kappaB signaling; negative regulation of anoikis; and regulation of gene expression. Located in cytosol and nucleoplasm. Part of beta-catenin-TCF complex. [provided by Alliance of Genome Resources, Apr 2022]
Function Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. Enhances FOXG1/BF-1- and HES1-mediated transcriptional repression (By similarity).
The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.
Cellular Localization Nucleus
Calculated MW 83.2 kDa
PTM Phosphoprotein, Ubl conjugation

Images (1) Click the Picture to Zoom In

  • ARG66945 anti-TLE1 antibody [SQab22275] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human synovial sarcoma stained with ARG66945 anti-TLE1 antibody [SQab22275]. Antigen Retrieval: Heat tissue section in Tris-EDTA buffer (pH 9.0).