anti-TLR3 antibody

anti-TLR3 antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Immune System antibody; Microbiology and Infectious Disease antibody; Signaling Transduction antibody


Product Description Rabbit Polyclonal antibody recognizes TLR3
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TLR3
Immunogen Synthetic peptide (15 aa) within aa. 780-830 of Human TLR3.
Conjugation Un-conjugated
Alternate Names Toll-like receptor 3; CD antigen CD283; CD283; IIAE2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFICC: 1 μg/ml; IF: 10 μg/ml
IHC-P1 - 10 μg/ml
WB1 - 2 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Daudi Cell Lysate


Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 142980 Mouse TLR3

GeneID: 7098 Human TLR3

Swiss-port # O15455 Human Toll-like receptor 3

Swiss-port # Q99MB1 Mouse Toll-like receptor 3

Gene Symbol TLR3
Gene Full Name toll-like receptor 3
Background TLR3 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR3 is known to recognize viral double-stranded (ds) RNA, a molecular pattern associated with viral infection. Recently it has been shown to recognize viruses such as Influenza A and West Nile Virus and can mediate entry of at least West Nile Virus. |
Highlight Related products:
TLR3 Antibody antibodies; Anti-Rabbit IgG secondary antibodies;
Related poster download:
Toll-like Receptor.pdf
Research Area Immune System antibody; Microbiology and Infectious Disease antibody; Signaling Transduction antibody
Calculated MW 104 kDa
PTM Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
TLR3 signaling requires a proteolytic cleavage mediated by cathepsins CTSB and CTSH, the cleavage occurs between amino acids 252 and 346. The cleaved form of TLR3 is the predominant form found in endosomes.

Images (3) Click the Picture to Zoom In

  • ARG54656 anti-TLR3 antibody ICC/IF image

    Immunocytochemistry: EL4 cells stained with ARG54656 anti-TLR3 antibody at 1 μg/ml.

  • ARG54656 anti-TLR3 antibody ICC/IF image

    Immunofluorescence: EL4 cells stained with ARG54656 anti-TLR3 antibody at 10 μg/ml.

  • ARG54656 anti-TLR3 antibody WB image

    Western blot: Daudi cell lysate stained with ARG54656 anti-TLR3 antibody at (A) 1 and (B) 2 μg/ml.

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Sample Loading Amount:15 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC