ARG54704

anti-TLR3 antibody

anti-TLR3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Immune System antibody; Microbiology and Infectious Disease antibody; Signaling Transduction antibody
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Overview

Product Description

Rabbit Polyclonal antibody recognizes TLR3

Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Target Name TLR3
Immunogen TLR3 recombinant protein.
Conjugation Un-conjugated
Alternate Names Toll-like receptor 3; CD antigen CD283; CD283; IIAE2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Purification Protein A purified
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7098 Human TLR3

Swiss-port # O15455 Human Toll-like receptor 3

Gene Symbol TLR3
Gene Full Name toll-like receptor 3
Background The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene. [provided by RefSeq, Jul 2008]
Function Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response. [From Uniprot]
Cellular Localization Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endosome membrane
Research Area Immune System antibody; Microbiology and Infectious Disease antibody; Signaling Transduction antibody
Calculated MW 104 kDa
PTM Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
TLR3 signaling requires a proteolytic cleavage mediated by cathepsins CTSB and CTSH, the cleavage occurs between amino acids 252 and 346. The cleaved form of TLR3 is the predominant form found in endosomes.

Images (2) Click the Picture to Zoom In

  • ARG54704 anti-TLR3 antibody WB image

    Western blot: 15 µg of Jurkat cell lysate stained with ARG54704 anti-TLR3 antibody at 1:1000 dilution.

  • ARG54704 anti-TLR3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse brain tissue stained with ARG54704 anti-TLR3 antibody.

Customer's Feedback

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Review for anti-TLR3 antibody

Application:WB

Sample:Jurkat

Sample Loading Amount:15 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC