anti-TPH2 phospho (Ser19) antibody
anti-TPH2 phospho (Ser19) antibody for Western blot and Rat
|Product Description||Rabbit Polyclonal antibody recognizes TPH2 phospho (Ser19)|
|Predict Reactivity||Ms, Bov, Zfsh|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser19 conjugated to KLH|
|Alternate Names||Tryptophan 5-hydroxylase 2; ADHD7; NTPH; Neuronal tryptophan hydroxylase; Tryptophan 5-monooxygenase 2; EC 188.8.131.52|
|Application Note||Specific for the ~55k tryptophan hydroxylase protein phosphorylated at Ser19.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Buffer||10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol|
|Stabilizer||0.1 mg/ml BSA, 50% Glycerol|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||tryptophan hydroxylase 2|
|Background||Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser19 which lies within the regulatory domain of TPH2 (McKinney et al., 2005).|
|Research Area||Neuroscience antibody|
|Calculated MW||56 kDa|
Images (1) Click the Picture to Zoom In
ARG52457 anti-TPH2 phospho (Ser19) antibody WB image
Western blot: Recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of Ca2+/calmodulin dependent kinase II (CaMKII) showing specific immunolabeling of the ~55 kDa tryptophan hydroxylase protein phosphorylated at Ser19 stained with ARG52457 anti-TPH2 phospho (Ser19) antibody.