anti-TPH2 phospho (Ser19) antibody

anti-TPH2 phospho (Ser19) antibody for Western blot and Rat

Neuroscience antibody


Product Description Rabbit Polyclonal antibody recognizes TPH2 phospho (Ser19)
Tested Reactivity Rat
Predict Reactivity Ms, Bov, Zfsh
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TPH2
Antigen Species Rat
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser19 conjugated to KLH
Conjugation Un-conjugated
Alternate Names Tryptophan 5-hydroxylase 2; ADHD7; NTPH; Neuronal tryptophan hydroxylase; Tryptophan 5-monooxygenase 2; EC

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note Specific for the ~55k tryptophan hydroxylase protein phosphorylated at Ser19.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 317675 Rat TPH2

Swiss-port # Q8CGU9 Rat Tryptophan 5-hydroxylase 2

Gene Symbol TPH2
Gene Full Name tryptophan hydroxylase 2
Background Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser19 which lies within the regulatory domain of TPH2 (McKinney et al., 2005).
Research Area Neuroscience antibody
Calculated MW 56 kDa

Images (1) Click the Picture to Zoom In

  • ARG52457 anti-TPH2 phospho (Ser19) antibody WB image

    Western blot: Recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of Ca2+/calmodulin dependent kinase II (CaMKII) showing specific immunolabeling of the ~55 kDa tryptophan hydroxylase protein phosphorylated at Ser19 stained with ARG52457 anti-TPH2 phospho (Ser19) antibody.