ARG52449

anti-TR2 antibody

anti-TR2 antibody for Western blot and Human,Mouse,Rat

Gene Regulation antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes TR2
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TR2
Antigen Species Mouse
Immunogen Fusion protein from the N-terminal region of mouse TR2
Conjugation Un-conjugated
Alternate Names Testicular receptor 2; Nuclear receptor subfamily 2 group C member 1; TR2; Orphan nuclear receptor TR2

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000
Application Note Specific for the ~64 kDa TR2 protein in Western blots of testes and nuclear extracts from MEL cell lines.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Neat Serum
Buffer Neat serum
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22025 Mouse NR2C1

GeneID: 252924 Rat NR2C1

GeneID: 7181 Human NR2C1

Gene Symbol NR2C1
Gene Full Name nuclear receptor subfamily 2, group C, member 1
Background Testicular receptor 2 (TR2) is a member of the orphan nuclear receptor family. It is widely expressed at a low level throughout the adult testis. TR2 represses transcription and binds DNA directly interacting with HDAC3 and HDAC4 via DNA-binding domains (Franco et al., 2003). TR2 has also been implicated in regulation of estrogen receptor activity in mammary glands (Hun et al., 2002). In addition, TR2 has recently been shown to form a heterodimer with TR4 that can bind to the direct repeat 6 element of the hepatitis B virus (HBV) enhancer II region thus suppressing HBV gene expression (Lin et al., 2008).
Research Area Gene Regulation antibody
Calculated MW 67 kDa
PTM Sumoylation requires both PIAS1 and UBE2I. Sumoylation appears to dissociate NR2C1 from the PML nuclear bodies. Enhances the interaction with NRIP1 but inhibits interaction with KAT2B. In proliferating cells, stimulation by all-trans retinoic acid, activation of MAPK1-mediated phosphorylation and recruitment to PML bodies with subsequent sumoylation, suppresses OCT4 expression (By similarity).
Phosphorylated on several serine and threonine residues. Phosphorylation on Thr-222, stimulated by all-trans retinoic acid (atRA) mediates PML location and sumoylation in proliferating cells which then modulates its association with effector molecules, KAT2B and NRIP1. Phosphorylation on Ser-581 by PKC is important for protein stability and function as activator of RARB (By similarity).

Images (1) Click the Picture to Zoom In

  • ARG52449 anti-TR2 antibody WB image

    Western Blot: rat testes lysate showing specific immunolabeling of the ~ 64k TR2 protein stained with TR2 antibody (ARG52449).