ARG53680

anti-TRADD antibody

anti-TRADD antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody

Overview

Product Description

Rabbit Polyclonal antibody recognizes TRADD

Tested Reactivity Hu
Tested Application IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TRADD
Antigen Species Human
Immunogen Synthetic peptide derived from C-terminus of human TRADD.
Conjugation Un-conjugated
Alternate Names TNFR1-associated DEATH domain protein; TNFRSF1A-associated via death domain; Hs.89862; Tumor necrosis factor receptor type 1-associated DEATH domain protein

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100
IPAssay-dependent
WB1 - 2 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Liver Carcinoma

Properties

Form Liquid
Purification Immunogen affinity purified
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8717 Human TRADD

Swiss-port # Q15628 Human Tumor necrosis factor receptor type 1-associated DEATH domain protein

Gene Symbol TRADD
Gene Full Name TNFRSF1A-associated via death domain
Background The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death signaling and NF-kappaB activation. This protein binds adaptor protein TRAF2, reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thus suppresses TRAF2 mediated apoptosis. This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway. [provided by RefSeq, Jul 2008]
Function The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A by TRIP12: acts by interacting with TRIP12, leading to disrupt interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A (By similarity). Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B. [UniProt]
Cellular Localization Cytoplasm
Highlight Related products:
TRADD antibodies; Anti-Rabbit IgG secondary antibodies;
Related poster download:
The NF-kappa B Pathways.pdf
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody
Calculated MW 34 kDa

Images (2) Click the Picture to Zoom In

  • ARG53680 anti-TRADD antibody WB image

    Western blot: 30 μg of HeLa cell 1) with no treatment, or 2) treat with 5 ng/ml TNF-a for 5 min. The blots were stained with ARG53680 anti-TRADD antibody at 1:500 dilution.

  • ARG53680 anti-TRADD antibody IHC-P image

    Immunohistochemistry: Human Liver Carcinoma stained with TRADD antibody (ARG53680)