anti-TRADD antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody
Rabbit Polyclonal antibody recognizes TRADD
|Tested Application||IHC-P, IP, WB|
|Immunogen||Synthetic peptide derived from C-terminus of human TRADD.|
|Alternate Names||TNFR1-associated DEATH domain protein; TNFRSF1A-associated via death domain; Hs.89862; Tumor necrosis factor receptor type 1-associated DEATH domain protein|
|Application Note||IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Positive Control||Liver Carcinoma|
|Purification||Immunogen affinity purified|
|Buffer||PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide|
|Preservative||< 0.1% Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||TNFRSF1A-associated via death domain|
|Background||The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death signaling and NF-kappaB activation. This protein binds adaptor protein TRAF2, reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thus suppresses TRAF2 mediated apoptosis. This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway. [provided by RefSeq, Jul 2008]|
|Function||The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A by TRIP12: acts by interacting with TRIP12, leading to disrupt interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A (By similarity). Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B. [UniProt]|
TRADD antibodies; Anti-Rabbit IgG secondary antibodies;
Related poster download:
The NF-kappa B Pathways.pdf
|Research Area||Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody|
|Calculated MW||34 kDa|
Images (2) Click the Picture to Zoom In
ARG53680 anti-TRADD antibody WB image
Western blot: 30 μg of HeLa cell 1) with no treatment, or 2) treat with 5 ng/ml TNF-a for 5 min. The blots were stained with ARG53680 anti-TRADD antibody at 1:500 dilution.
ARG53680 anti-TRADD antibody IHC-P image
Immunohistochemistry: Human Liver Carcinoma stained with TRADD antibody (ARG53680)