ARG63260
anti-TRIM2 antibody
anti-TRIM2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Cell Biology and Cellular Response antibody; Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes TRIM2 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Predict Reactivity | Ms, Cow, Dog |
| Tested Application | FACS, IHC-P, WB |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_056086.2; NP_001123539.1). Cross-reactivity with TRIM3 has been demonstrated. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TRIM2 |
| Antigen Species | Human |
| Immunogen | DSGNHCFKVYRYLQ |
| Conjugation | Un-conjugated |
| Alternate Names | RNF86; EC 6.3.2.-; RING finger protein 86; Tripartite motif-containing protein 2; CMT2R; E3 ubiquitin-protein ligase TRIM2 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # D3ZQG6 Rat Tripartite motif-containing protein 2 Swiss-port # Q9C040 Human Tripartite motif-containing protein 2 |
|---|---|
| Background | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. Its function has not been identified. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| Calculated MW | 82 kDa |
| PTM | RING-type zinc finger-dependent and UBE2D1-dependent autoubiquitination. |
Images (3) Click the Picture to Zoom In
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ARG63260 anti-TRIM2 antibody WB image
Western Blot: Rat Brain lysate (35 µg protein in RIPA buffer) stained with ARG63260 anti-TRIM2 antibody at 0.5 µg/ml dilution.
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ARG63260 anti-TRIM2 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Cortex. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63260 anti-TRIM2 antibody at 6.3 µg/ml dilution followed by AP-staining.
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ARG63260 anti-TRIM2 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed Jurkat cells permeabilized with 0.5% Triton. Cells were stained with ARG63260 anti-TRIM2 antibody (blue line) at 10 µg/ml dilution and overnight, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
