ARG54665

anti-TRIM71 antibody

anti-TRIM71 antibody for ELISA,Immunohistochemistry,Western blot,ICC/IF and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes TRIM71
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, ICC/IF, IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TRIM71
Immunogen Synthetic peptide (15 aa) within aa. 680-730 of Human TRIM71.
Conjugation Un-conjugated
Alternate Names EC 6.3.2.-; Tripartite motif-containing protein 71; Protein lin-41 homolog; LIN-41; LIN41; E3 ubiquitin-protein ligase TRIM71

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-Dependent
ICC/IF20 μg/mL
IHC5 μg/mL
WB1 - 2 μg/mL
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human Brain Tissue Lysate

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 131405 Human TRIM71

GeneID: 301042 Rat TRIM71

GeneID: 636931 Mouse TRIM71

Gene Symbol TRIM71
Gene Full Name tripartite motif containing 71, E3 ubiquitin protein ligase
Background TRIM71 Antibody: TRIM71 is the mammalian ortholog to the C. elegans heterochronic protein Lin41, a protein that is thought to be important in postembryonic development, and is genetically and biochemically downstream of both Shh and Fgf signaling pathways. Similar to its C. elegans homolog, TRIM71 is a target of the microRNA let-7 and is likely to play a role in mammalian development. Recent experiments have indicated that TRIM71 is an E3 ubiquitin ligase and can interact with the Dicer and the Argonaute proteins Ago1, Ago2, and Ago4. Overexpression and depletion of TRIM71 led to inverse changes in Ago2 protein levels, suggesting TRIM71 can regulate Ago2 turnover. Finally, TRIM71 cooperates with the pluripotency factor Lin-28 in regulating let-7. |
Research Area Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody
Calculated MW 93 kDa
PTM Autoubiquitinated.

Images (3) Click the Picture to Zoom In

  • ARG54665 anti-TRIM71 antibody ICC/IF image

    Immunofluorescence: human brain cells stained with ARG54665 anti-TRIM71 antibody at 20 μg/ml.

  • ARG54665 anti-TRIM71 antibody IHC image

    Immunohistochemistry: human brain tissue stained with ARG54665 anti-TRIM71 antibody at 5 μg/ml.

  • ARG54665 anti-TRIM71 antibody WB image

    Western blot: human brain tissue lysate stained with ARG54665 anti-TRIM71 antibody at (A) 1 and (B) 2 μg/ml.