ARG45818

anti-TRMT2B antibody

anti-TRMT2B antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes TRMT2B
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TRMT2B
Antigen Species Human
Immunogen Recombinant protein containing to human TRMT2B.
Conjugation Un-conjugated
Alternate Names TRMT2B; TRNA Methyltransferase 2 Homolog B; CXorf34; TRNA (Uracil-5-)-Methyltransferase Homolog B; RRNA (Uracil-5-)-Methyltransferase TRMT2B; TRM2 Homolog B; EC 2.1.1.35  47; FLJ12687; TRNA Methyltransferase 2 Homolog B (S. Cerevisiae); TRNA (Uracil(54)-C(5))-Methyltransferase Homolog; TRNA (Uracil-5-)-Methyltransferase Homolog; TRM2 TRNA Methyltransferase 2 Homolog B; Chromosome X Open Reading Frame 34; TRM2 Homolog; DJ341D10.3; EC 2.1.1.-

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
IHC-P2-5 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 47 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 215201 Mouse TRMT2B

GeneID: 79979 Human TRMT2B

Swiss-port # Q8BQJ6 Mouse tRNA (uracil(54)-C(5))-methyltransferase homolog

Swiss-port # Q96GJ1 Human tRNA (uracil(54)-C(5))-methyltransferase homolog

Gene Symbol TRMT2B
Gene Full Name TRNA Methyltransferase 2 Homolog B
Background This gene encodes a homolog of the TRM2 gene in S. cerevisiae. The yeast gene encodes a tRNA methyltransferase that plays a role in tRNA maturation. The yeast protein also has endo-exonuclease activity and may be involved in DNA double strand break repair. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Nov 2009]
Function Mitochondrial S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine in tRNAs and 12S rRNA. [UniProt]
Cellular Localization Mitochondrion. [UniProt]
Calculated MW 56 kDa

Images (5) Click the Picture to Zoom In

  • ARG45818 anti-TRMT2B antibody IHC-P image

    Immunohistochemistry: Human colon cancer stained with ARG45818 anti-TRMT2B antibody at 2 μg/ml dilution.

  • ARG45818 anti-TRMT2B antibody WB image

    Western blot: HepG2 stained with ARG45818 anti-TRMT2B antibody at 0.5 μg/ml dilution.

  • ARG45818 anti-TRMT2B antibody FACS image

    Flow Cytometry: Daudi stained with ARG45818 anti-TRMT2B antibody at 1 µg/10^6 cells dilution.

  • ARG45818 anti-TRMT2B antibody WB image

    Western blot: Rat heart stained with ARG45818 anti-TRMT2B antibody at 0.5 μg/ml dilution.

  • ARG45818 anti-TRMT2B antibody WB image

    Western blot: Mouse heart stained with ARG45818 anti-TRMT2B antibody at 0.5 μg/ml dilution.