ARG45574

anti-TRMT61A antibody

anti-TRMT61A antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes TRMT61A
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TRMT61A
Antigen Species Human
Immunogen Recombinant protein containing to human TRMT61A.
Conjugation Un-conjugated
Alternate Names TRMT61A; TRNA Methyltransferase 61A; C14orf172; Gcd14p; HTRM61; GCD14; MRNA Methyladenosine-N(1)-Methyltransferase Catalytic Subunit TRMT61A; TRNA (Adenine(58)-N(1))-Methyltransferase Catalytic Subunit TRMT61A; TRNA(M1A58)-Methyltransferase Subunit TRMT61A; TRNA(M1A58)MTase Subunit TRMT61A; FLJ40452; TRM61; TRNA (Adenine-N(1)-)-Methyltransferase Catalytic Subunit TRMT61A; TRNA (Adenine-N(1)-)-Methyltransferase Catalytic Subunit TRM61; TRNA Methyltransferase 61 Homolog A (S. Cerevisiae); TRNA(M1A58)-Methyltransferase Subunit TRM61; Chromosome 14 Open Reading Frame 172; TRNA Methyltransferase 61 Homolog A; TRNA(M1A58)MTase Subunit TRM61; EC 2.1.1.220; EC 2.1.1.36; EC 2.1.1.-

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
IHC-P1-2 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 31 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 115708 Human TRMT61A

GeneID: 314462 Rat TRMT61A

GeneID: 328162 Mouse TRMT61A

Gene Symbol TRMT61A
Gene Full Name TRNA Methyltransferase 61A
Background Enables mRNA (adenine-N1-)-methyltransferase activity. Involved in mRNA processing. Part of tRNA (m1A) methyltransferase complex. [provided by Alliance of Genome Resources, Feb 2025]
Function Catalytic subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Catalytic subunit of mRNA N(1)-methyltransferase complex, which mediates methylation of adenosine residues at the N(1) position of a small subset of mRNAs: N(1) methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries . [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 31 kDa
PTM Acetylation; Phosphoprotein. [UniProt]

Images (5) Click the Picture to Zoom In

  • ARG45574 anti-TRMT61A antibody IHC-P image

    Immunohistochemistry: Human ovarian serous cancer stained with ARG45574 anti-TRMT61A antibody at 2 μg/ml dilution.

  • ARG45574 anti-TRMT61A antibody WB image

    Western blot: PC-3 stained with ARG45574 anti-TRMT61A antibody at 0.5 μg/ml dilution.

  • ARG45574 anti-TRMT61A antibody FACS image

    Flow Cytometry: PC-3 stained with ARG45574 anti-TRMT61A antibody at 1 µg/10^6 cells dilution.

  • ARG45574 anti-TRMT61A antibody IHC-P image

    Immunohistochemistry: Rat spleen stained with ARG45574 anti-TRMT61A antibody at 2 μg/ml dilution.

  • ARG45574 anti-TRMT61A antibody IHC-P image

    Immunohistochemistry: Mouse spleen stained with ARG45574 anti-TRMT61A antibody at 2 μg/ml dilution.