ARG45258
anti-TRMT61B antibody
anti-TRMT61B antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Polyclonal antibody recognizes TRMT61B |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TRMT61B |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human TRMT61B. |
| Conjugation | Un-conjugated |
| Alternate Names | TRMT61B; TRNA Methyltransferase 61B; TRNA (Adenine(58)-N(1))-Methyltransferase, Mitochondrial; MRNA Methyladenosine-N(1)-Methyltransferase; FLJ20628; Potential TRNA (Adenine(58)-N(1))-Methyltransferase Catalytic Subunit TRMT61B; Potential TRNA (Adenine-N(1)-)-Methyltransferase Catalytic Subunit TRMT61B; TRNA Methyltransferase 61 Homolog B (S. Cerevisiae); TRNA Methyltransferase 61 Homolog B; EC 2.1.1.220; EC 2.1.1. |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 57 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9BVS5 Human tRNA (adenine(58)-N(1))-methyltransferase, mitochondrial |
|---|---|
| Gene Symbol | TRMT61B |
| Gene Full Name | TRNA Methyltransferase 61B |
| Background | Enables mRNA (adenine-N1-)-methyltransferase activity; rRNA (adenine) methyltransferase activity; and tRNA (adenine(58)-N1)-methyltransferase activity. Involved in mRNA processing and mitochondrial tRNA processing. Located in mitochondrial matrix. [provided by Alliance of Genome Resources, Nov 2024] |
| Function | Methyltransferase that catalyzes the formation of N1-methyladenine at position 58 (m1A58) in various tRNAs in mitochondrion, including tRNA(Leu) (deciphering codons UUA or UUG), tRNA(Lys) and tRNA(Ser) (deciphering codons UCA, UCU, UCG or UCC) (. [UniProt] |
| Cellular Localization | Mitochondrion. [UniProt] |
| Calculated MW | 53 kDa |
Images (3) Click the Picture to Zoom In
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ARG45258 anti-TRMT61B antibody IHC-P image
Immunohistochemistry: Human colorectal adenocarcinoma stained with ARG45258 anti-TRMT61B antibody at 2 μg/ml dilution.
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ARG45258 anti-TRMT61B antibody WB image
Western blot: HepG2, Hela, and A549 stained with ARG45258 anti-TRMT61B antibody at 0.5 μg/ml dilution.
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ARG45258 anti-TRMT61B antibody FACS image
Flow Cytometry: 293T stained with ARG45258 anti-TRMT61B antibody at 1 µg/10^6 cells dilution.
