ARG51301

anti-TRPC6 antibody

anti-TRPC6 antibody for Western blot and Human,Mouse,Rat

Signaling Transduction antibody
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Overview

Product Description

Rabbit Polyclonal antibody recognizes TRPC6

Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TRPC6
Antigen Species Human
Immunogen peptide Corresponding to the amino terminal sequence derived from mouse TRPC6.
Conjugation Un-conjugated
Alternate Names FSGS2; Transient receptor protein 6; TrpC6; Short transient receptor potential channel 6; TRP-6; TRP6

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22068 Mouse TRPC6

GeneID: 7225 Human TRPC6

Swiss-port # Q61143 Mouse Short transient receptor potential channel 6

Swiss-port # Q9Y210 Human Short transient receptor potential channel 6

Gene Symbol Trpc6
Gene Full Name transient receptor potential cation channel, subfamily C, member 6
Background The protein encoded by this gene forms a receptor-activated calcium channel in the cell membrane. The channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of this channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in this gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2).
Function Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion. [UniProt]
Research Area Signaling Transduction antibody
Calculated MW 106 kDa
PTM Phosphorylated by FYN, leading to an increase of TRPC6 channel activity.

Images (2) Click the Picture to Zoom In

  • ARG51301 anti-TRPC6 antibody WB image

    Western blot: 20 µg of A549 cell lysates stained with ARG51301 anti-TRPC6 antibody at 1:500 dilution.

  • ARG51301 anti-TRPC6 antibody WB image

    Western blot: 20 µg of Mouse brain and Rat brain lysates stained with ARG51301 anti-TRPC6 antibody at 1:500 dilution.

Customer's Feedback

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Review for anti-TRPC6 antibody

Application:WB

Sample:Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-TRPC6 antibody

Application:WB

Sample:A549

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC