ARG51301
anti-TRPC6 antibody
anti-TRPC6 antibody for Western blot and Human,Mouse,Rat
Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TRPC6 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TRPC6 |
| Antigen Species | Human |
| Immunogen | peptide Corresponding to the amino terminal sequence derived from mouse TRPC6. |
| Conjugation | Un-conjugated |
| Alternate Names | FSGS2; Transient receptor protein 6; TrpC6; Short transient receptor potential channel 6; TRP-6; TRP6 |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q61143 Mouse Short transient receptor potential channel 6 Swiss-port # Q9Y210 Human Short transient receptor potential channel 6 |
|---|---|
| Gene Symbol | Trpc6 |
| Gene Full Name | transient receptor potential cation channel, subfamily C, member 6 |
| Background | The protein encoded by this gene forms a receptor-activated calcium channel in the cell membrane. The channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of this channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in this gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2). |
| Function | Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion. [UniProt] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 106 kDa |
| PTM | Phosphorylated by FYN, leading to an increase of TRPC6 channel activity. |
Images (2) Click the Picture to Zoom In
-
ARG51301 anti-TRPC6 antibody WB image
Western blot: 20 µg of A549 cell lysates stained with ARG51301 anti-TRPC6 antibody at 1:500 dilution.
-
ARG51301 anti-TRPC6 antibody WB image
Western blot: 20 µg of Mouse brain and Rat brain lysates stained with ARG51301 anti-TRPC6 antibody at 1:500 dilution.
Customer's Feedback
Good
Review for anti-TRPC6 antibody
Application：WB
Sample：Mouse brain and Rat brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-TRPC6 antibody
Application：WB
Sample：A549
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
