ARG46620

anti-TYW1 antibody

anti-TYW1 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes TYW1
Tested Reactivity Hu, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TYW1
Antigen Species Human
Immunogen A 19 amino acid synthetic peptide within the last 50 amino acids of human TYW1.
Conjugation Un-conjugated
Alternate Names tRNA-yW synthesizing protein 1 homolog (S. cerevisiae); TYW1; TYW1A; RSAFD1; YPL207W; S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase; Radical S-adenosyl methionine and flavodoxin domain-containing protein 1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity chromatography purified
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -670°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 55253 Human TYW1

Swiss-port # Q9NV66 Human S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase

Gene Symbol TYW1
Gene Full Name tRNA-yW synthesizing protein 1 homolog (S. cerevisiae)
Background Wybutosine (yW) is a hypermodified guanosine found in phenylalanine tRNA adjacent to the anticodon that stabilizes codon-anticodon interactions in the ribosome. In yeast, the homolog of this gene is essential for the synthesis of wybutosine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
Function Probable component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine, an intermediate in wybutosine biosynthesis (By similarity). [UniProt]
Calculated MW 84 kDa