anti-TdT antibody [SQab20183]
anti-TdT antibody [SQab20183] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
|Product Description||Recombinant Rabbit Monoclonal antibody [SQab20183] recognizes TdT|
|Tested Application||IHC-P, WB|
|Immunogen||Synthetic peptide within aa. 1-100 of Human TdT.|
|Alternate Names||EC 184.108.40.206; Terminal addition enzyme; Terminal transferase; DNA nucleotidylexotransferase; Terminal deoxynucleotidyltransferase; TDT|
|Application Note||IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Positive Control||Thymoma tissue.|
|Purification||Purification with Protein A.|
|Buffer||PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.|
|Preservative||0.01% Sodium azide|
|Stabilizer||40% Glycerol and 0.05% BSA|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||DNA nucleotidylexotransferase|
|Background||This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]|
|Function||Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. [UniProt]|
|Cellular Localization||Nucleus. [UniProt]|
|Calculated MW||59 kDa|
Images (2) Click the Picture to Zoom In
ARG66750 anti-TdT antibody [SQab20183] WB image (Customer's Feedback)
Western blot: JurKat and Molt-4 cell lysate stained with ARG66750 anti-TdT antibody [SQab20183] at 1:1000 dilution.
ARG66750 anti-TdT antibody [SQab20183] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human thymoma tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66750 anti-TdT antibody [SQab20183] at 18°C - 25°C for 30 minutes.
Review for anti-TdT antibody [SQab20183]
Sample Loading Amount：20 μg
SDS Page Type：Denatured
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：HRP