anti-Thymidine Kinase antibody [3B3.E11]

anti-Thymidine Kinase antibody [3B3.E11] for ELISA,Western blot and Human

Gene Regulation antibody


Product Description Mouse Monoclonal antibody [3B3.E11] recognizes Thymidine Kinase
Tested Reactivity Hu
Tested Application ELISA, WB
Specificity This antibody specifically recognizes human thymidine kinase.
Host Mouse
Clonality Monoclonal
Clone 3B3.E11
Isotype IgG1
Target Name Thymidine Kinase
Antigen Species Human
Immunogen 5’-His-tagged recombinanthuman thymidine kinase
Conjugation Un-conjugated
Alternate Names Thymidine kinase, cytosolic; TK2; EC

Application Instructions

Application Note Western blot: antibody at 10 ug/ml will recognize TK in 5, 10, or 20 ug lysates of cells that express TK.
ELISA: use antibody at 2 - 5 ug/ml (optimized for TK on solid phase at 10 ug/ml).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Protein G-purified
Buffer PBS (pH 7.4)
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 7083 Human TK1

Swiss-port # P04183 Human Thymidine kinase, cytosolic

Gene Symbol TK1
Gene Full Name thymidine kinase 1, soluble
Background Thymidine kinase (TK) belongs to agroup of enzymes such as dihydrofolatereductase, thymidylate synthase, andDNA polymerase that are involved inDNA synthesis and precursorproduction. High levels of theseenzymes are present in proliferatingcells, and low levels are found in restingcells, due to multiple regulatorymechanisms that ensure exclusiveexpression of these enzymes inreplicating cells. TK is responsible forcatalyzing the phosphorylation ofthymidine, which functions as a part ofthe pyrimidine salvage pathway involvedin DNA synthesis. The activities ofenzymes such as TK are essential forthe activation of severalchemotherapeutically importantnucleoside analogues that areadministered as prodrugs.
Research Area Gene Regulation antibody
Calculated MW 25 kDa
PTM Phosphorylated on Ser-13 in mitosis.

Clone References

Mechanism of action of the Aurora kinase inhibitor CCT129202 and in vivo quantification of biological activity.

Chan F et al.
Mol Cancer Ther.,  (2007)




Fluorodeoxyuridine modulates cellular expression of the DNA base excision repair enzyme uracil-DNA glycosylase.

WB / Human

Fischer JA et al.
Cancer Res.,  (2006)




IKK alpha regulates estrogen-induced cell cycle progression by modulating E2F1 expression.

Tu Z et al.
J Biol Chem.,  (2006)




Isolation and characterization of Dictyostelium thymidine kinase 1 as a calmodulin-binding protein.

WB / Dictyostelium discoideum

O'Day DH et al.
Biochem Biophys Res Commun.,  (2005)