ARG66635

anti-Tpo / Thrombopoietin antibody [SQab19153]

anti-Tpo / Thrombopoietin antibody [SQab19153] for IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab19153] recognizes Tpo / Thrombopoietin
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SQab19153
Isotype IgG
Target Name Tpo / Thrombopoietin
Antigen Species Human
Immunogen Synthetic peptide within aa. 833-933 of Human Tpo / Thrombopoietin.
Conjugation Un-conjugated
Full Name thrombopoietin
Alternate Names C-mpl ligand; Thrombopoietin; MPLLG; TPO; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand; MKCSF; THCYT1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C - 25°C) for 30 minutes.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 38 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 7066 Human THPO

Swiss-port # P40225 Human Thrombopoietin

Gene Symbol THPO
Gene Full Name thrombopoietin
Background Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014]
Function Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. [UniProt]
Cellular Localization Secreted. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG66635 anti-Tpo / Thrombopoietin antibody [SQab19153] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human thyroid tissue stained with ARG66635 anti-Tpo / Thrombopoietin antibody [SQab19153]. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0).