ARG20177

anti-TrkB antibody

anti-TrkB antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Chicken,Human,Mouse,Rat

Overview

Product Description

Rabbit Polyclonal antibody recognizes TrkB

Tested Reactivity Hu, Ms, Rat, Chk
Tested Application ICC/IF, IHC-P, IP, WB
Specificity Trk protein exists as variably glycosylated entities with the major forms having molecular weights of 140 kDa, 110 kDa, and the unglycosylated form of 80 kDa.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TrkB
Antigen Species Human
Immunogen Synthetic peptide surrounding amino acid 810 of human TrkB
Conjugation Un-conjugated
Full Name neurotrophic tyrosine kinase, receptor, type 2
Alternate Names TRKB; Neurotrophic tyrosine kinase receptor type 2; Trk-B; trk-B; Tropomyosin-related kinase B; TrkB tyrosine kinase; BDNF/NT-3 growth factors receptor; GP145-TrkB; EC 2.7.10.1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF5-10 µg/ml
IHC-PAssay-dependent.
IPAssay-dependent.
WB0.5-4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HL-60 cell lysate
Calculated MW 92 kDa

Properties

Form Liquid
Purification Affinity Purified Antibody
Buffer PBS, 30% Glycerol, 0.5% BSA and 0.01% Thimerosal
Preservative 0.01% Thimerosal
Stabilizer 30% Glycerol, 0.5% BSA
Concentration 0.2 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 18212 Mouse NTRK2

GeneID: 25054 Rat NTRK2

GeneID: 396157 Chicken NTRK2

Gene Symbol NTRK2
Gene Full Name neurotrophic tyrosine kinase, receptor, type 2
Background Due to the various splice variants, the trk proteins exist as variably glycosylated entities with the major forms having molecular weights of 140-145 kDa, 110 kDa and the unglycosylated form of 80 kDa. trkB contains 33.3% carbohydrate by weight representing modification on 10 of 12 N-glycosylation sites. The primary ligand for trkB is BDNF which induces the phosphorylation of the protein and subsequent binding of PLC-gamma via SH2 domains. TrkB may function to modulate neuronal responses to the neurotrophins acting through trkB such as BDNF.
Function Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG20177 anti-TrkB antibody WB image

    Western Blot: HL-60 cell lysate stained with anti-TrkB antibody (ARG20177).