ARG10590

anti-Trypsin antibody [A16-N]

anti-Trypsin antibody [A16-N] for Western blot and Human

Overview

Product Description Rabbit Monoclonal antibody [A16-N] recognizes Trypsin
Tested Reactivity Hu
Tested Application WB
Host Rabbit
Clonality Monoclonal
Clone A16-N
Isotype IgG
Target Name Trypsin
Antigen Species Human
Immunogen Synthetic peptide around the N-terminus of Human pancreatic cationic trypsin
Conjugation Un-conjugated
Full Name protease, serine, 1 (trypsin 1)
Alternate Names Trypsin I; TRY4; TRY1; Serine protease 1; EC 3.4.21.4; Cationic trypsinogen; Trypsin-1; TRYP1; TRP1; Beta-trypsin

Application Instructions

Application Suggestion
Tested Application Dilution
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 20 mM Tris-HCl (pH 8.0), 0.05% Sodium azide and 10 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 10 mg/ml BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 5644 Human PRSS1

Swiss-port # P07477 Human Trypsin-1

Gene Symbol PRSS1
Gene Full Name protease, serine, 1 (trypsin 1)
Background This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7. [provided by RefSeq, Jul 2008]
Function Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates. [UniProt]
PTM Occurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122.
Sulfation at Tyr-154 increases selectivity towards basic versus apolar residues at the P2' position of inhibitors that bind in a substrate-like fashion. Although the increase in selectivity is relatively small, it may facilitate digestion of a broader range of dietary proteins.

Images (1) Click the Picture to Zoom In

  • ARG10590 anti-Trypsin antibody [A16-N] WB image

    Western blot: 20 ng, 40 ng, 100 ng, and 200 ng of Human trypsin stained with ARG10590 anti-Trypsin antibody [A16-N].