ARG66337

anti-Type II Cytokeratins antibody [SQab1885]

anti-Type II Cytokeratins antibody [SQab1885] for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human

Signaling Transduction antibody; Carcinoma Marker antibody

Overview

Product Description Recombinant Rabbit Monoclonal antibody [SQab1885] recognizes Type II Cytokeratins
Tested Reactivity Hu
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1885
Isotype IgG
Target Name Type II Cytokeratins
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 300-400 of Human Type-II Cytokeratins.
Conjugation Un-conjugated
Full Name keratin 1, type II
Alternate Names 67 kDa cytokeratin; KRT1A; EPPK; Keratin, type II cytoskeletal 1; EHK1; K1; Keratin-1; CK1; Cytokeratin-1; Hair alpha protein; Type-II keratin Kb1; CK-1; NEPPK; EHK

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
IHC-P1:1600 - 1:3200
WB1:5000 - 1:10000
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 51 - 66 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3848 Human KRT1

Swiss-port # P04264 Human Keratin, type II cytoskeletal 1

Gene Symbol KRT1
Gene Full Name keratin 1, type II
Background The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
Function May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK. [UniProt]
Highlight Related products:
Anti-Rabbit IgG secondary antibodies;
Related news:
Cancer Pathology Markers (SQ clones)
Research Area Signaling Transduction antibody; Carcinoma Marker antibody
PTM Undergoes deimination of some arginine residues (citrullination). [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG66337 anti-Type II Cytokeratins antibody [SQab1885] FACS image

    Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66337 anti-Type II Cytokeratins antibody [SQab1885] (red) at 1:50 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.

  • ARG66337 anti-Type II Cytokeratins antibody [SQab1885] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human liver tissue stained with ARG66337 anti-Type II Cytokeratins antibody [SQab1885] at 1:1,600 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.

  • ARG66337 anti-Type II Cytokeratins antibody [SQab1885] WB image

    Western blot: 10 µg of A549, SW480, HaCat and Caco-2 cell lysates stained with ARG66337 anti-Type II Cytokeratins antibody [SQab1885] at 1:10000 dilution.