ARG52464

anti-Tyrosine Hydroxylase phospho (Ser40) antibody

anti-Tyrosine Hydroxylase phospho (Ser40) antibody for Western blot,ICC/IF,Immunohistochemistry and Human,Mouse,Rat,Mammal

Cancer antibody; Metabolism antibody; Neuroscience antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Tyrosine Hydroxylase phospho (Ser40)
Tested Reactivity Hu, Ms, Rat, Mamm
Tested Application ICC/IF, IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Tyrosine Hydroxylase
Antigen Species Rat
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser40 conjugated to KLH
Conjugation Un-conjugated
Alternate Names DYT14; TYH; Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase; TH; DYT5b; EC 1.14.16.2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000
IHCfrozen sections: 1:1000
WB1:1000
Application Note Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser40. Some higher molecular weight bands may be detected by the antibody depending upon the brain region being studied, protein loads and the detection methods used. The antibody has three orders of magnitude selectivity over dephospho TH.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21823 Mouse TH

GeneID: 25085 Rat TH

GeneID: 7054 Human TH

Gene Symbol TH
Gene Full Name tyrosine hydroxylase
Background Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines Dopamine and Norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982; Haycock et al., 1992; Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.
Highlight Related Antibody Duos and Panels:
ARG30212 Phospho Tyrosine Hydroxylase Antibody Panel (Total, pS31, pS40)
Related products:
Tyrosine Hydroxylase antibodies; Tyrosine Hydroxylase Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Astrocyte-to-neuron conversion for Parkinson's disease treatment
Research Area Cancer antibody; Metabolism antibody; Neuroscience antibody
Calculated MW 59 kDa

Images (2) Click the Picture to Zoom In

  • ARG52464 anti-Tyrosine Hydroxylase phospho (Ser40) antibody WB image

    Western blot: Recombinant phospho- and dephospho-Tyrosine Hydroxylase. The blots were stained with anti-pan Tyrosine Hydroxylase antibody and ARG52464 anti-Tyrosine Hydroxylase phospho (Ser40) antibody.

  • ARG52464 anti-Tyrosine Hydroxylase phospho (Ser40) antibody IHC image

    Immunohistochemistry: Light-stimulated Rabbit retina stained with ARG52464 anti-Tyrosine Hydroxylase phospho (Ser40) antibody showing labeling of TH when phosphorylated at Ser40.