ARG45766

anti-UBA5 antibody

anti-UBA5 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes UBA5
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name UBA5
Antigen Species Human
Immunogen Recombinant protein containing to human UBA5.
Conjugation Un-conjugated
Alternate Names UBA5; Ubiquitin Like Modifier Activating Enzyme 5; UBE1DC1; Ubiquitin-Activating Enzyme E1 Domain-Containing Protein 1; Ubiquitin-Activating Enzyme E1-Domain Containing 1; Ubiquitin-Like Modifier-Activating Enzyme 5; Ubiquitin-Activating Enzyme 5; UFM1-Activating Enzyme; FLJ23251; UBA5, Ubiquitin-Activating Enzyme E1 Homolog (Yeast); UBA5, Ubiquitin-Activating Enzyme E1 Homolog; Ubiquitin-Like Modifier Activating Enzyme 5; EIEE44; SCAR24; THIFP1; ThiFP1; DEE44

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
IHC-P2-5 μg/ml
WB0.1-0.25 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 50 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 300968 Rat UBA5

GeneID: 66663 Mouse UBA5

GeneID: 79876 Human UBA5

Gene Symbol UBA5
Gene Full Name Ubiquitin Like Modifier Activating Enzyme 5
Background This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been identified on chromosome 1. [provided by RefSeq, Feb 2016]
Function E1-like enzyme which specifically catalyzes the first step in ufmylation. [UniProt]
Cellular Localization Cytoplasm; Endoplasmic reticulum; Golgi apparatus; Membrane; Nucleus. [UniProt]
Calculated MW 45 kDa
PTM Phosphoprotein. [UniProt]

Images (5) Click the Picture to Zoom In

  • ARG45766 anti-UBA5 antibody IHC-P image

    Immunohistochemistry: Human breast cancer stained with ARG45766 anti-UBA5 antibody at 2 μg/ml dilution.

  • ARG45766 anti-UBA5 antibody WB image

    Western blot: CACO-2 stained with ARG45766 anti-UBA5 antibody at 0.5 μg/ml dilution.

  • ARG45766 anti-UBA5 antibody FACS image

    Flow Cytometry: PC-3 stained with ARG45766 anti-UBA5 antibody at 1 µg/10^6 cells dilution.

  • ARG45766 anti-UBA5 antibody WB image

    Western blot: Rat pancreas stained with ARG45766 anti-UBA5 antibody at 0.5 μg/ml dilution.

  • ARG45766 anti-UBA5 antibody WB image

    Western blot: Mouse pancreas stained with ARG45766 anti-UBA5 antibody at 0.5 μg/ml dilution.