anti-ULK1 / ATG1 antibody

anti-ULK1 / ATG1 antibody for ICC/IF,Western blot and Human

Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody


Product Description Rabbit Polyclonal antibody recognizes ULK1 / ATG1
Tested Reactivity Hu
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ULK1 / ATG1
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 642-672 of Human ULK1 / ATG1.
Conjugation Un-conjugated
Alternate Names ATG1A; Unc51.1; Autophagy-related protein 1 homolog; ATG1; Unc-51-like kinase 1; EC; hATG1; UNC51; Serine/threonine-protein kinase ULK1

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control A2058


Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 8408 Human ULK1

Swiss-port # O75385 Human Serine/threonine-protein kinase ULK1

Gene Symbol ULK1
Gene Full Name unc-51 like autophagy activating kinase 1
Function Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. [UniProt]
Cellular Localization Cytoplasm, cytosol. Preautophagosomal structure. Note=Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 113 kDa
PTM Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.

Images (2) Click the Picture to Zoom In

  • ARG54811 anti-ULK1 / ATG1 antibody ICC/IF image

    Immunofluorescence: U251 cells were treated with Chloroquine (50 µM, 16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). U251 cells were then stained with ARG54811 anti-ULK1 / ATG1 antibody (green) at 1:100 dilution, 2 h at room temperature. Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). ULK1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

  • ARG54811 anti-ULK1 / ATG1 antibody WB image

    Western blot: A2058 cell lysate stained with ARG54811 anti-ULK1 / ATG1 antibody.