ARG46627

anti-ULK2 antibody

anti-ULK2 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes ULK2
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ULK2
Antigen Species Human
Immunogen A 17 amino acid synthetic peptide within the last 50 amino acids of human ULK2.
Conjugation Un-conjugated
Alternate Names unc-51-like kinase 2 (C. elegans); ULK2; ATG1B; Unc51.2; KIAA0623; Unc-51-like kinase 2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 100 kDa

Properties

Form Liquid
Purification Affinity chromatography purified
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -677°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 9706 Human ULK2

Swiss-port # Q8IYT8 Human Serine/threonine-protein kinase ULK2

Gene Symbol ULK2
Gene Full Name unc-51-like kinase 2 (C. elegans)
Background This gene encodes a protein that is similar to a serine/threonine kinase in C. elegans which is involved in axonal elongation. The structure of this protein is similar to the C. elegans protein in that both proteins have an N-terminal kinase domain, a central proline/serine rich (PS) domain, and a C-terminal (C) domain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Dec 2008]
Function Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons. [UniProt]
Cellular Localization Cytoplasm; Cytoplasmic vesicle; Cytoplasmic vesicle membrane. [UniProt]
Calculated MW 105, 114 kDa
PTM Phosphoprotein. [UniProt]