ARG54630

anti-Ubiquitin antibody

anti-Ubiquitin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoelectrophoresis,Immunoprecipitation,Western blot and Bovine,Chicken,D. melanogaster ,Dog,E. coli,Fish,Guinea pig,Hamster,Human,Monkey,Mouse,Pig,Rabbit,Rat,S. cerevisiae,Sheep,Xenopus laevis,Yeast

Cell Biology and Cellular Response antibody; Gene Regulation antibody; Neuroscience antibody
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Overview

Product Description

Rabbit Polyclonal antibody recognizes Ubiquitin

Tested Reactivity Hu, Ms, Rat, Bov, Chk, Dm, Dog, E. coli, Fsh, Gpig, Hm, Mk, Pig, Rb, S. cerevisiae, Sheep, Xenopus laevis, Yeast
Tested Application ICC/IF, IE, IHC-Fr, IHC-P, IP, WB
Specificity This antibody will identify free ubiquitin aswell as ubiquinated proteins on western blot.It recognizes human, monkey, mouse, rat,hamster, rabbit guinea pig, bovine, porcine,canine, sheep, chicken, Xenopus, yeast,Drosophila, and fish ubiquitin.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Ubiquitin
Antigen Species Bovine
Immunogen Native bovine ubiquitin conjugated to KLH.
Conjugation Un-conjugated
Alternate Names Polyubiquitin-B

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IE1:1000
IHC-Fr1:200
IHC-PAssay-dependent
IP1:100
WB1:1000 - 1:5000
Application Note IHC-Fr: For PFA perfusion fixed frozen section.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Heat-shocked HeLa and Mouse brain

Properties

Form Liquid
Purification Protein A-purified antibody
Buffer PBS (pH 7.2), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22187 Mouse UBB

GeneID: 281370 Bovine UBB

GeneID: 7314 Human UBB

Gene Symbol UBB
Gene Full Name ubiquitin B
Background Ubiquitin is a highly-conserved, 76-aminoacid polypeptide expressed in all eukaryoticcells. Ubiquitination of proteins facilitatestargeting of cytosolic and nuclear proteinsfor degradation by proteasomes. Thisessential cellular process involves a multienzymecascade of ubiquitin-activatingenzymes (E1s), ubiquitin-conjugatingenzymes (E2s or Ubcs), and ubiquitinproteinligases (E3s). Ubiquitination alsoparticipates in internalization anddegradation of plasma membrane proteinssuch as T cell receptor subunits while stillassociated with membranes of theendoplasmic reticulum.
Function Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. [UniProt]
Highlight Related products:
Ubiquitin antibodies; Ubiquitin ELISA Kits; Ubiquitin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody; Neuroscience antibody
Calculated MW 26 kDa
PTM Ubiquitin: Phosphorylated at Ser-65 by PINK1 during mitophagy. Phosphorylated ubiquitin specifically binds and activates parkin (PRKN), triggering mitophagy (PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:25527291). Phosphorylation does not affect E1-mediated E2 charging of ubiquitin but affects discharging of E2 enzymes to form polyubiquitin chains. It also affects deubiquitination by deubiquitinase enzymes such as USP30 (PubMed:25527291).

Images (1) Click the Picture to Zoom In

  • ARG54630 anti-Ubiquitin antibody WB image

    Western Blot: Heat-shocked HeLa cell lysate stained with ARG54630 anti-Ubiquitin antibody.

Customer's Feedback

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Review for anti-Ubiquitin antibody

Application:WB

Sample:Mouse brain and Rat brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Pro-Prion is a Membrane Adaptor Protein for E3 Ligase C-Cbl to Ubiquitinate IGF-IR Promoting Melanoma Metastasis

WB / Human

Huan Li et al.
SSRN.,  (2022)

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CD2-associated Protein Contributes to Hepatitis C Virus Propagation and Steatosis by Disrupting Insulin Signaling.

WB / Human

Zhang Huixia et al.
Hepatology.,  (2018)

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