ARG20178

anti-VDAC1 / Porin antibody

anti-VDAC1 / Porin antibody for Immunohistochemistry,Western blot and Bovine,Human,Mouse,Rabbit,Rat

Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
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anti-VDAC1 / Porin antibody

Rabbit Polyclonal antibody recognizes VDAC1 / Porin

ARG20178 Arigo biolaboratories
4.0 out of 5 based on 3
anti-VDAC1 / Porin antibody is rated 4.0 out of 5 by 3.

anti-VDAC1 / Porin antibody

Overview

Product Description

Rabbit Polyclonal antibody recognizes VDAC1 / Porin

Tested Reactivity Hu, Ms, Rat, Bov, Rb
Tested Application IHC, WB
Specificity The antibody recognizes ~31 kDa VDAC1 / Porin from samples of human, mouse, rat, bovine, pig, and rabbit origins.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name VDAC1 / Porin
Antigen Species Human
Immunogen Synthetic peptide surrounding amino acid 189 of human VDAC1
Conjugation Un-conjugated
Alternate Names Plasmalemmal porin; PORIN; Outer mitochondrial membrane protein porin 1; Voltage-dependent anion-selective channel protein 1; hVDAC1; Porin 31HL; Porin 31HM; VDAC-1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC2.5 µg/ml
WB0.5-4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Western Blot: 3T3 cell lysate. IHC: Liver tissue
Calculated MW 31 kDa

Properties

Form Liquid
Purification Affinity Purified Antibody
Buffer PBS, 30% Glycerol, 0.5% BSA and 0.01% Thimerosal
Preservative 0.01% Thimerosal
Stabilizer 30% Glycerol, 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 100008751 Rabbit VDAC1

GeneID: 22333 Mouse VDAC1

GeneID: 282119 Bovine VDAC1

Background The Voltage-Dependent Anion Channel (VDAC or mitochondrial Porin)I is an outer membrane mitochondrial protein. The VDAC protein is thought to form the major pores through which adenine nucleotides are transferred through the outer mitochondrial membrane. VDAC has also been implicated in the formation of the mitochondrial permeability transition pore complex in apoptotic cells. This complex, formed by VDAC, ANT, and CypD is thought to allow the mitochondria to undergo metabolic uncoupling and irreversible morphologic changes that ultimately destroy the mitochondria during apoptosis.
Highlight Related products:
VDAC1 antibodies; Anti-Rabbit IgG secondary antibodies;
Related poster download:
The Structure & Functions of Mitochondria.pdf
Research Area Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
PTM Phosphorylation at Ser-193 by NEK1 promotes the open conformational state preventing excessive mitochondrial membrane permeability and subsequent apoptotic cell death after injury. Phosphorylation by the AKT-GSK3B axis stabilizes the protein probably by preventing ubiquitin-mediated proteasomal degradation.
Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.

Images (1) Click the Picture to Zoom In

  • ARG20178 anti-VDAC1 / Porin antibody WB image

    Western blot: 30 µg of HT-29 cell lysate stained with ARG20178 anti-VDAC1 / Porin antibody at 1:250 dilution.

Customer's Feedback

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Review for anti-VDAC1 / Porin antibody

Application:WB

Sample:HT29, Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1 µg/ml

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

Review for anti-VDAC1 / Porin antibody

Application:WB

Sample:Rat liver

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:250

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

Review for anti-VDAC1 / Porin antibody

Application:WB

Sample:Mouse liver

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:250

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC