ARG22249

anti-VDAC1 / Porin antibody [S152B-23]

anti-VDAC1 / Porin antibody [S152B-23] for ICC/IF,Immunohistochemistry,Western blot and Human,Mouse,Rat

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Overview

Product Description Mouse Monoclonal antibody [S152B-23] recognizes VDAC1 / Porin
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC, WB
Specificity Detects ~30kDa. Does not cross-react with VDAC2 or VDAC3 (based on KO validation results).
Host Mouse
Clonality Monoclonal
Clone S152B-23
Isotype IgG2a
Target Name VDAC1 / Porin
Antigen Species Human
Immunogen Full-length (aa. 1-283) fusion protein of Human VDAC1.
Mouse: 98% identity (279/283 around aa. identical).
Rat: 98% identity (279/283 around aa. identical); >60% identity with VDAC2 and VDAC3.
Conjugation Un-conjugated
Alternate Names Plasmalemmal porin; PORIN; Outer mitochondrial membrane protein porin 1; Voltage-dependent anion-selective channel protein 1; hVDAC1; Porin 31HL; Porin 31HM; VDAC-1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IHCAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.1% Sodium azide and 50% Glycerol
Preservative 0.1% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 22333 Mouse VDAC1

GeneID: 7416 Human VDAC1

GeneID: 83529 Rat VDAC1

Gene Symbol VDAC1
Gene Full Name voltage-dependent anion channel 1
Background This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.[provided by RefSeq, Sep 2010]
Function Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis. [UniProt]
Cellular Localization Cell membrane, Mitochondrion, Mitochondrion outer membrane
Calculated MW 31 kDa
PTM Phosphorylation at Ser-193 by NEK1 promotes the open conformational state preventing excessive mitochondrial membrane permeability and subsequent apoptotic cell death after injury. Phosphorylation by the AKT-GSK3B axis stabilizes the protein probably by preventing ubiquitin-mediated proteasomal degradation.
Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.

Images (2) Click the Picture to Zoom In

  • ARG22249 anti-VDAC1 / Porin antibody [S152B-23] WB image

    Western blot: 20 µg of MCF7 cell lysate stained with ARG22249 anti-VDAC1 / Porin antibody [S152B-23] at 1:1000 dilution.

  • ARG22249 anti-VDAC1 / Porin antibody [S152B-23] ICC/IF image

    Immunofluorescence: Human Neuroblastoma cell line SK-N-BE. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: ARG22249 anti-VDAC1 / Porin antibody [S152B-23] at 1:100 for 60 min at RT. Secondary Antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ARG22249 anti-VDAC1 / Porin antibody [S152B-23] (D) Composite.

Customer's Feedback

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Review for anti-VDAC1 / Porin antibody [S152B-23]

Application:WB

Sample:Mouse brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC