ARG10658

anti-VEGF antibody

anti-VEGF antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes VEGF
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name VEGF
Antigen Species Human
Immunogen Synthetic peptide around the N-terminus of Human VEGF (VDIFQEYPDEIEYIFK). (100% homologous in Human, Mouse and Rat).
Conjugation Un-conjugated
Full Name vascular endothelial growth factor A
Alternate Names MVCD1; Vascular permeability factor; VEGF-A; VPF; VEGF; Vascular endothelial growth factor A

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P0.5 - 1 µg/ml
WB0.5 - 1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 27 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.025% Sodium azide and 2.5% BSA.
Preservative 0.025% Sodium azide
Stabilizer 2.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 22339 Mouse VEGFA

GeneID: 7422 Human VEGFA

GeneID: 83785 Rat VEGFA

Gene Symbol VEGFA
Gene Full Name vascular endothelial growth factor A
Background This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008]
Function Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG10658 anti-VEGF antibody IHC-P image

    Immunohistochemistry: Human lung cancer tissue stained with ARG10658 anti-VEGF antibody.

  • ARG10658 anti-VEGF antibody WB image

    Western blot: 1) 10 ng, 2) 5 ng, and 3) 2.5 ng of recombinant Human protein stained with ARG10658 anti-VEGF antibody.