ARG10513

anti-VEGF antibody [VG-1]

anti-VEGF antibody [VG-1] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Dog,Human,Mouse,Rabbit,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
publication_link Publication5

Overview

Product Description

Mouse Monoclonal antibody [VG-1] recognizes VEGF

Tested Reactivity Hu, Ms, Rat, Dog, Rb
Species Does Not React With Cow
Tested Application ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
Specificity Detects the 121, 165 and 189 VEGF isoforms in fixed specimens.
Host Mouse
Clonality Monoclonal
Clone VG-1
Isotype IgG1
Target Name VEGF
Immunogen Recombinant VEGF 189 protein.
Conjugation Un-conjugated
Full Name Vascular endothelial growth factor A
Alternate Names MVCD1; Vascular permeability factor; VEGF-A; VPF; VEGF; Vascular endothelial growth factor A

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent.
ICC/IF5 µg/ml
IHC-FrAssay-dependent.
IHC-PAssay - dependent
IP1:1000
WB5 - 10 µg/ml
Application Note WB: Suggested blocking buffer: 3% milk blocking for 1 hr at RT (better than at 4°C, overnight). Recommend primary and secondary dilution buffer: TBST.
IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0 (recommended) or citrate buffer pH6.0.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Corpus luteum, VEGF165 recombinant protein, astrocytoma or hemoangiosarcoma.
Calculated MW 19-22 kDa

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 22339 Mouse VEGFA

GeneID: 7422 Human VEGFA

GeneID: 83785 Rat VEGFA

Gene Symbol VEGFA
Gene Full Name Vascular endothelial growth factor A
Background VEGF is an angiogenic growth factor and a prognostic indicator for cancer when detected in serum. VG-1 is useful for studies of VEGF and angiogenesis in human pathological material.
Function Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. [Uniprot]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody

Specific References

Yougui pills exert osteoprotective effects on rabbit steroid-related osteonecrosis of the femoral head by activating β-catenin.

IHC-P / Rabbit

Zhang Peng et al.
Biomed Pharmacother.,  (2019)

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Long noncoding RNA CPS1-IT1 suppresses melanoma cell metastasis through inhibiting Cyr61 via competitively binding to BRG1.

WB / Human

Zhou Xiaobo et al.
J Cell Physiol.,  (2019)

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Astragaloside IV inhibits lung cancer progression and metastasis by modulating macrophage polarization through AMPK signaling

IHC-P / Mouse

Xu Fei et al.
J Exp Clin Cancer Res.,  (2018)

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Plant galactolipid dLGG suppresses lung metastasis of melanoma through deregulating TNF-α-mediated pulmonary vascular permeability and circulating oxylipin dynamics in mice.

WB / Mouse

Yang Chung-Chih et al.
Int J Cancer.,  (2018)

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An EGLN1 mutation may regulate hypoxic response in cyanotic congenital heart disease through the PHD2/HIF-1A pathway

WB / Human

Zhou Yuanlin et al.
Genes & Diseases,  (2018)

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