ARG45501

anti-VLCAD antibody

anti-VLCAD antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes VLCAD
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name VLCAD
Antigen Species Human
Immunogen Synthetic peptide corresponding to C-terminal region of human VLCAD.
Conjugation Un-conjugated
Alternate Names ACADVL; Acyl-CoA Dehydrogenase Very Long Chain; VLCAD; LCACD; ACAD6; Very Long-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial; Acyl-Coenzyme A Dehydrogenase, Very Long Chain; EC 1.3.8.9; EC 1.3.99 47

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC/IF5 μg/ml
IHC-P2-5 μg/ml
IP0.5-2 μg/ml
WB0.1-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 70 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11370 Mouse ACADVL

GeneID: 25363 Rat ACADVL

GeneID: 37 Human ACADVL

Gene Symbol ACADVL
Gene Full Name Acyl-CoA Dehydrogenase Very Long Chain
Background The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Function Very long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats [UniProt]
Cellular Localization Membrane; Mitochondrion; Mitochondrion inner membrane. [UniProt]
Calculated MW 70 kDa
PTM Acetylation; Phosphoprotein; S-nitrosylation. [UniProt]

Images (8) Click the Picture to Zoom In

  • ARG45501 anti-VLCAD antibody IHC-P image

    Immunohistochemistry: Human liver cancer stained with ARG45501 anti-VLCAD antibody at 2 μg/ml dilution.

  • ARG45501 anti-VLCAD antibody ICC/IF image

    Immunofluorescence: U2OS stained with ARG45501 anti-VLCAD antibody at 5 μg/ml dilution.

  • ARG45501 anti-VLCAD antibody WB image

    Western blot: Hela stained with ARG45501 anti-VLCAD antibody at 0.5 μg/ml dilution.

  • ARG45501 anti-VLCAD antibody IP image

    Immunoprecipitation: Hela lysate immunoprecipitated with 2 μg ARG45501 anti-VLCAD antibody.

  • ARG45501 anti-VLCAD antibody FACS image

    Flow Cytometry: U251 stained with ARG45501 anti-VLCAD antibody at 1 µg/10^6 cells dilution.

  • ARG45501 anti-VLCAD antibody IHC-P image

    Immunohistochemistry: Rat heart stained with ARG45501 anti-VLCAD antibody at 2 μg/ml dilution.

  • ARG45501 anti-VLCAD antibody WB image

    Western blot: Rat heart stained with ARG45501 anti-VLCAD antibody at 0.5 μg/ml dilution.

  • ARG45501 anti-VLCAD antibody WB image

    Western blot: Mouse liver stained with ARG45501 anti-VLCAD antibody at 0.5 μg/ml dilution.