ARG46791

anti-Venus antibody

anti-Venus antibody for ICC/IF,Western blot and Human

Overview

Product Description Goat Polyclonal antibody recognizes Venus
Tested Reactivity Hu
Tested Application ICC/IF, WB
Specificity In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody also recognizes Venus/GFP and does not cross-react to mCherry/red fluorescent proteins.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Venus
Immunogen Purified recombinant GFP peptide produced in E. coli.
Conjugation Un-conjugated
Alternate Names GFP, Green fluorescent protein

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:1000
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Epitope affinity purified
Buffer PBS, 0.05% Sodium azide and 20% Glycerol.
Preservative 0.05% Sodium azide
Stabilizer 20% Glycerol
Concentration 3 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Venus Fluorescent Protein
Background Goat polyclonal antibody to Venus. Venus is a genetic mutant of green fluorescent protein (GFP), derived from Aequorea victoria. Its excitation/emission peaks are 515/528 nm respectively and is often used as a donor-acceptor pair by researchers in FRET based co-localization studies.

Images (2) Click the Picture to Zoom In

  • ARG46791 anti-Venus antibody ICC/IF image

    Immunofluorescence: 293HEK stained with ARG46791 anti-Venus antibody at 1:50 dilution.

  • ARG46791 anti-Venus antibody WB image

    Western blot: Venus Rab7 stained with ARG46791 anti-Venus antibody at 1:2000 dilution.