ARG11100

anti-Vimentin antibody [SQab1859] (FITC)

anti-Vimentin antibody [SQab1859] (FITC) for Flow cytometry,ICC/IF,IHC-Frozen sections,Western blot and Chicken,Dog,Hamster,Human,Monkey,Mouse,Pig,Rat,Xenopus,Zebrafish

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [SQab1859] recognizes Vimentin
Tested Reactivity Hu, Ms, Rat, Chk, Dog, Hm, Mk, Pig, Xenopus, Zfsh
Tested Application FACS, ICC/IF, IHC-Fr, WB
Specificity This antibody reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchyme derived tumors e.g. lymphoma, sarcoma and melanoma.
Host Mouse
Clonality Monoclonal
Clone SQab1859
Isotype IgG1
Target Name Vimentin
Antigen Species Bovine
Immunogen A cytoskeletal vimentin extract of calf lens.
Conjugation FITC
Alternate Names Vimentin; CTRCT30; HEL113

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10
ICC/IF1:10
IHC-Fr1:10
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.09% Sodium azide and 0.1% BSA.
Preservative 0.09% Sodium azide
Stabilizer 0.1% BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 100522394 Pig VIM

GeneID: 22352 Mouse VIM

GeneID: 407747 Pig VIM

Gene Symbol VIM
Gene Full Name vimentin
Background This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]
Cellular Localization Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix. [UniProt]
Highlight Related products:
Vimentin antibodies; Vimentin Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
New antibody panels for Myofibroblasts and CAFs
Calculated MW 54 kDa
PTM Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.

O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.

S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG11100 anti-Vimentin antibody [SQab1859] (FITC) IHC-Fr image

    Immunofluorescence: Frozen section of Human colon tissue stained with ARG11100 anti-Vimentin antibody [SQab1859] (FITC).

    Note reactivity in connective tissue while epithelial cells remain negative.