ARG52470

anti-Visinin like 1 antibody [2D11]

anti-Visinin like 1 antibody [2D11] for ICC/IF,IHC-Frozen sections,Western blot and Bovine,Human,Mouse,Rat

Cancer antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [2D11] recognizes Visinin like 1
Tested Reactivity Hu, Ms, Rat, Bov
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 2D11
Isotype IgG1
Target Name Visinin like 1
Antigen Species Human
Immunogen Recombinant human VSNL1 purified from E. coli
Conjugation Un-conjugated
Alternate Names HPCAL3; HUVISL1; Visinin-like protein 1; HLP3; Hippocalcin-like protein 3; VILIP; VLP-1; VILIP-1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:500 - 1:1000
IHC-Fr1:500 - 1:1000
WB1:500 - 1:1000
Application Note Specific for the ~22k protein
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer PBS and 10 mM Sodium azide
Preservative 10 mM Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 26950 Mouse VSNL1

GeneID: 282124 Bovine VSNL1

GeneID: 7447 Human VSNL1

Gene Symbol VSNL1
Gene Full Name visinin-like 1
Background Visinin-like protein 1 (VSNL1), also known as VILIP1, is a calcium sensor protein expressed exclusively in neurons. Highest levels of VSNL1 expression are found in cerebellar Purkinje cells. VSNL1 has been implicated in the modulation of cell signaling cascades via regulation of adenyl cyclase activity (Braunewell et al., 1997). Additionally, VSNL1 has been associated with amyloid plaques and neurofibrillar tangles in Alzheimer’s disease (Schnurra et al., 2001).
Research Area Cancer antibody; Signaling Transduction antibody
Calculated MW 22 kDa

Images (4) Click the Picture to Zoom In

  • ARG52470 anti-Visinin like 1 antibody [2D11] WB image

    Western blot: Rat cerebellum lysate showing specific immunolabeling of the ~ 22k VSNL1 protein stained with ARG52470 anti-Visinin like 1 antibody [2D11].

  • ARG52470 anti-Visinin like 1 antibody [2D11] IHC-Fr image

    Immunohistochemistry: Frozen section of Rat cerebellum tissue stained with ARG52470 anti-Visinin like 1 antibody [2D11] (red) at 1:500 dilution, and costained with anti-GFAP antibody (green) at 1:5000 dilution. DAPI (blue) for nuclear staining. Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies.

    Clone 2D11 reveals protein expressed in granule cell membranes and in synapses in the white matter and molecular layers of the cerebellum. The GFAP antibody stains the processes of Bergmann glia and astroglia.

  • ARG52470 anti-Visinin like 1 antibody [2D11] IHC image

    Immunohistochemistry: Rat cerebellum stained with ARG52470 anti-Visinin like 1 antibody [2D11] showing strong synaptic staining of VSNL1 (green) in the molecular layer (ML) and MAP2 stained with ARG52328 anti-MAP2 antibody in red.

  • ARG52470 anti-Visinin like 1 antibody [2D11] WB image

    Western blot: Rat brain, Rat cerebellum and Mouse brain lysates stained with ARG52470 anti-Visinin like 1 antibody [2D11] (green) at 1:1000 dilution.